Effects of vascular endothelial growth factor (VEGF) on MC3T3-E1

Tan, Ying Ying, Yang, Yan-Qi, Chai, Lei, Wong, Ricky W.K. and Rabie, A. Bakr M. (2010) Effects of vascular endothelial growth factor (VEGF) on MC3T3-E1. Orthodontics & Craniofacial Research, 13 4: 223-228. doi:10.1111/j.1601-6343.2010.01498.x

Author Tan, Ying Ying
Yang, Yan-Qi
Chai, Lei
Wong, Ricky W.K.
Rabie, A. Bakr M.
Title Effects of vascular endothelial growth factor (VEGF) on MC3T3-E1
Journal name Orthodontics & Craniofacial Research   Check publisher's open access policy
ISSN 1601-6335
Publication date 2010-11
Sub-type Article (original research)
DOI 10.1111/j.1601-6343.2010.01498.x
Volume 13
Issue 4
Start page 223
End page 228
Total pages 6
Place of publication Malden, MA, U.S.A.
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Objective: To investigate whether VEGF has direct effects on bone cells activities and to better understand how VEGF promotes bone remodeling.

Materials and Methods: MC3T3-E1 cell line was cultured with and without VEGF in vitro. The cells in both control and test groups were collected at different culture time points of 24, 48 and 72 h. Real-time polymerase chain reaction (qPCR) was carried out to quantify the mRNA expression of VEGF receptor (VEGFR2), alkaline phosphatase (ALP) and osteocalcin (OCN), osteoprotegerin (OPG) and receptor activator of nuclear factor kappa b ligand (RANKL).

Results: The expression of VEGFR2 significantly increased by 53% at 24 h and remained increased by 8% at 72 h compared to control (p < 0.05). ALP showed an early increase by 73% at 24 h (p < 0.001), but dropped by 14 and 41% at 48 and 72 h, respectively (p < 0.05). OCN was down-regulated by 41% at 24 h but then up-regulated by 149% at 72 h (p < 0.001). The expression of OPG significantly decreased by 7% at 24 h (p < 0.001) while dramatically increased by 133% at 72 h (p < 0.001). RANKL remained unchanged at all three time points (p > 0.05).

VEGF promotes bone remodeling by direct effects on steoblastic cells via regulating gene expression of ALP, OCN, and OPG through VEGFR2 signaling pathway.
Keyword Bone remodeling
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
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