An innovative microplate assay to facilitate the detection of antimicrobial activity in plant extracts

Sultanbawa, Y., Cusack, A., Currie, M. and Davis, C. (2009) An innovative microplate assay to facilitate the detection of antimicrobial activity in plant extracts. Journal of Rapid Methods and Automation in Microbiology, 17 4: 519-534.


Author Sultanbawa, Y.
Cusack, A.
Currie, M.
Davis, C.
Title An innovative microplate assay to facilitate the detection of antimicrobial activity in plant extracts
Journal name Journal of Rapid Methods and Automation in Microbiology   Check publisher's open access policy
ISSN 1060-3999
Publication date 2009-12
Sub-type Article (original research)
DOI 10.1111/j.1745-4581.2009.00187.x
Volume 17
Issue 4
Start page 519
End page 534
Total pages 16
Place of publication United States
Publisher Wiley-Blackwell Publishing, Inc.
Language eng
Formatted abstract A microplate assay was modified for the detection of antimicrobial activity in plant extracts. The aim was to develop an in vitro assay that could rapidly screen plant extracts to provide quantitative data on inhibition of microbial growth. A spectrophotometric assay using a microplate with serial dilutions of the plant extract and the bacteria was developed. Two bacteria, Staphylococcus aureus and Escherichia coli, were used for this study. Essential oils, oregano (Origanum vulgare) and lemon myrtle (Backhousia citriodora), and three active components carvacrol, thymol and citral were evaluated. The reproducibility of the assay was high, with correlation coefficients (r2) of the replicates of lemon myrtle and oregano with S. aureus and E. coli between 0.9321 and 0.9816. Similarly, r2 values for carvacrol, thymol and citral were between 0.8455 and 0.9814. This assay could also be used to measure antimicrobial activity in plant extracts which vary in pH and color.
Keyword Minimum Inhibitory Concentration
Plate-Based Assay
Essential Oils
Antibacterial Activity
Salmonella-Typhimurium
Listeria-Monocytogenes
Backhousia-Citriodora
Bacteria
Carvacrol
Products
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
ERA 2012 Admin Only
 
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