A rapid saponification procedure for the simultaneous HPLC determination of the natural levels of vitamins A, E and beta-carotene in milk

Hewavitharana, A. K. and Van Brakel, A. S. (1998) A rapid saponification procedure for the simultaneous HPLC determination of the natural levels of vitamins A, E and beta-carotene in milk. Milchwissenschaft-Milk Science International, 53 1: 11-15.

Author Hewavitharana, A. K.
Van Brakel, A. S.
Title A rapid saponification procedure for the simultaneous HPLC determination of the natural levels of vitamins A, E and beta-carotene in milk
Journal name Milchwissenschaft-Milk Science International   Check publisher's open access policy
ISSN 0026-3788
Publication date 1998
Sub-type Article (original research)
Volume 53
Issue 1
Start page 11
End page 15
Total pages 5
Place of publication Kempten, Germany
Publisher A V A Verlag Allgaeu
Language eng
Formatted abstract
A rapid saponification procedure was developed for the simultaneous extraction of vitamins A, E and β-carotene in milk, prior to HPLC analysis. A high accuracy, sensitivity and ruggedness were achieved by the use of an internal standard, β-tocopherol, which enabled the determination of the low levels of vitamins occurring naturally. Vitamin losses during extraction and the subsequent HPLC analysis were minimized by the use of antioxidants and by preconditioning the silica column with α-tocopherol in order to prevent irreversible adsorption of vitamin E on the column. The results compared well with those obtained from a non-saponification approach using a modified Röse-Gottlieb extraction. Relative standard deviations for repeatability of 4.9, 3.5 and 3.3% were achieved for vitamins A, E and β-carotene respectively. The rapid, single extraction and simultaneous HPLC analysis used for the 3 vitamins made the method well suited for routine analytical work.
Keyword Chromatographic Determination
Foods
Tocopherols
Products
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 2 times in Thomson Reuters Web of Science Article | Citations
Google Scholar Search Google Scholar
Created: Sat, 10 Sep 2011, 16:39:55 EST by System User on behalf of School of Pharmacy