Low-frequency spectroscopic analysis of monomeric and fibrillar lysozyme

Zakaria, Hidayatul A., Fischer, Bernd M., Bradley, Andrew P., Jones, Inke, Abbott, Derek, Middelberg, Anton P. J. and Falconer, Robert J. (2011) Low-frequency spectroscopic analysis of monomeric and fibrillar lysozyme. Applied Spectroscopy, 65 3: 260-264. doi:10.1366/10-06162

Author Zakaria, Hidayatul A.
Fischer, Bernd M.
Bradley, Andrew P.
Jones, Inke
Abbott, Derek
Middelberg, Anton P. J.
Falconer, Robert J.
Title Low-frequency spectroscopic analysis of monomeric and fibrillar lysozyme
Journal name Applied Spectroscopy   Check publisher's open access policy
ISSN 0003-7028
Publication date 2011-03
Sub-type Article (original research)
DOI 10.1366/10-06162
Volume 65
Issue 3
Start page 260
End page 264
Total pages 5
Place of publication Frederick, MD, United States
Publisher Society for Applied Spectroscopy
Collection year 2012
Language eng
Formatted abstract
Terahertz time-domain spectroscopy (THz-TDS) and Fourier transform infrared (FT-IR) spectroscopy were used to generate far-infrared and lowfrequency spectral measurements of monomeric lysozyme and lysozyme fibrils. The formation of lysozyme fibrils was verified by the Thioflavin T assay and transmission electron microscopy (TEM). It was evident in the FT-IR spectra that between 150 and 350 cm–1 the two spectra diverge, with the lysozyme fibrils showing higher absorbance intensity than the monomeric form. The broad absorption phenomenon is likely due to light scattered from the fibrillar architecture of lysozyme fibrils as supported by simulation of Rayleigh light scattering. The lack of discrete phonon-like peaks suggest that far-infrared spectroscopy cannot detect vibrational modes between the highly ordered hydrogen-bonded beta-pleated sheets of the lysozyme subunit.
Keyword Terahertz
Time-domain spectroscopy
Fourier transform infrared spectroscopy
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

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