Differential expression of 14-3-3 isoforms in human alcoholic brain

Mackay, Rachel K., Colson, Natalie J., Dodd, Peter R. and Lewohl, Joanne M. (2011) Differential expression of 14-3-3 isoforms in human alcoholic brain. Alcoholism: Clinical and Experimental Research, 35 6: 1041-1049. doi:10.1111/j.1530-0277.2011.01436.x


Author Mackay, Rachel K.
Colson, Natalie J.
Dodd, Peter R.
Lewohl, Joanne M.
Title Differential expression of 14-3-3 isoforms in human alcoholic brain
Journal name Alcoholism: Clinical and Experimental Research   Check publisher's open access policy
ISSN 0145-6008
1530-0277
Publication date 2011-06
Sub-type Article (original research)
DOI 10.1111/j.1530-0277.2011.01436.x
Volume 35
Issue 6
Start page 1041
End page 1049
Total pages 9
Place of publication Hoboken, NJ, United States
Publisher Wiley-Blackwell
Collection year 2012
Language eng
Formatted abstract
Background: Neuropathological damage as a result of chronic alcohol abuse often results in the impairment of cognitive function. The damage is particularly marked in the frontal cortex. The 14-3-3 protein family consists of 7 proteins,β, γ, ε, ζ, η, θ, and σ, encoded by 7 distinct genes. They are highly conserved molecular chaperones with roles in the regulation of metabolism, signal transduction, cell-cycle control, protein trafficking, and apoptosis. They may also play an important role in neurodegeneration in chronic alcoholism.
Methods: We used real-time PCR to measure the expression of 14-3-3 mRNA transcripts in both the dorsolateral prefrontal cortex and motor cortex of human brains obtained at autopsy.
Results: We found significantly lower 14-3-3β, γ, and θ expression in both cortical areas of alcoholics, but no difference in 14-3-3η expression, and higher expression of 14-3-3σ in both areas. Levels of 14-3-3ζ and ε transcripts were significantly lower only in alcoholic motor cortex.
Conclusions: Altered 14-3-3 expression could contribute to synaptic dysfunction and altered neurotransmission in chronic alcohol misuse by human subjects.

Keyword Alcohol abuse
Gene expression
Ethanol
Real-time PCR
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Chemistry and Molecular Biosciences
 
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