Selectively inducing the synthesis of a key structural exopolysaccharide in aerobic granules by enriching for Candidatus “Competibacter phosphatis”

Seviour, Thomas William, Lambert, Lynette K., Pijuan, Maite and Yuan, Zhiguo (2011) Selectively inducing the synthesis of a key structural exopolysaccharide in aerobic granules by enriching for Candidatus “Competibacter phosphatis”. Applied Microbiology and Biotechnology, 92 6: 1297-1305. doi:10.1007/s00253-011-3385-1


Author Seviour, Thomas William
Lambert, Lynette K.
Pijuan, Maite
Yuan, Zhiguo
Title Selectively inducing the synthesis of a key structural exopolysaccharide in aerobic granules by enriching for Candidatus “Competibacter phosphatis”
Formatted title
Selectively inducing the synthesis of a key structural exopolysaccharide in aerobic granules by enriching for Candidatus “Competibacter phosphatis”
Journal name Applied Microbiology and Biotechnology   Check publisher's open access policy
ISSN 0175-7598
1432-0614
Publication date 2011-12
Sub-type Article (original research)
DOI 10.1007/s00253-011-3385-1
Volume 92
Issue 6
Start page 1297
End page 1305
Total pages 9
Place of publication Heidelberg, Germany
Publisher Springer
Collection year 2012
Language eng
Formatted abstract
A gel-forming exopolysaccharide was previously shown to play an important structural role in aerobic granules treating nutrient-rich industrial wastewater. To identify whether this exopolysaccharide performs a similar role in other granular biomass and if conditions favouring its production can be more precisely elucidated, extracellular polymeric substances (EPS) were extracted from granules grown under four different operating conditions. 1H nuclear magnetic resonance (NMR) spectroscopy of their EPS indicated that the gel-forming exopolysaccharide was expressed in two granular sludges both enriched in Candidatus “Competibacter phosphatis”. In contrast, it was not expressed in granules performing denitrification with methanol as a carbon source and nitrate as the electron acceptor or granules enriched in Candidatus “Accumulibacter phosphatis” performing enhanced biological phosphorus removal from synthetic wastewater. In one of the first two sludges, the exopolysaccharide contained in the seeding granular sludge continued to be a major component of the granule EPS while Competibacter was being enriched. In the second sludge, a floccular sludge not containing the gel-forming exopolysaccharide initially was also enriched for Competibacter. In this sludge, an increase in particle size was detected
coinciding with a yield increase of EPS. NMR spectroscopy confirmed its yield increase to be attributable to the production of this structural gel-forming exopolysaccharide. The results show that (1) the particular gel-forming exopolysaccharide previously identified is not necessarily a key structural exopolysaccharide for all granule types, and (2) synthesis of this exopolysaccharide is induced under conditions favouring the selective enrichment of Competibacter. This indicates that Competibacter may be involved in its production.
Keyword Aerobic sludge granules
Exopolysaccharides
EPS
NMR
Competibacter
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Centre for Advanced Imaging Publications
Advanced Water Management Centre Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 14 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 14 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 07 Sep 2011, 12:56:25 EST by Sandrine Ducrot on behalf of Centre for Advanced Imaging