Transcriptome profiling of lung schistosomula, in vitro cultured schistosomula and adult Schistosoma japonicum

Chai, M., McManus, D. P., McInnes, R., Moertel, L., Tran, M., Loukas, A., Jones, M. K. and Gobert, G. N. (2006) Transcriptome profiling of lung schistosomula, in vitro cultured schistosomula and adult Schistosoma japonicum. Cellular and Molecular Life Sciences, 63 7-8: 919-929. doi:10.1007/s00018-005-5578-1


Author Chai, M.
McManus, D. P.
McInnes, R.
Moertel, L.
Tran, M.
Loukas, A.
Jones, M. K.
Gobert, G. N.
Title Transcriptome profiling of lung schistosomula, in vitro cultured schistosomula and adult Schistosoma japonicum
Journal name Cellular and Molecular Life Sciences   Check publisher's open access policy
ISSN 1420-682X
1420-9071
Publication date 2006-04
Sub-type Article (original research)
DOI 10.1007/s00018-005-5578-1
Volume 63
Issue 7-8
Start page 919
End page 929
Total pages 11
Place of publication Basel, Switzerland
Publisher Birkhaeuser Verlag
Language eng
Abstract The schistosomulum is the main target of vaccine-induced protective immunity; however, most studies have utilized schistosomula produced by mechanical transformation of infective larvae followed by in vitro culture rather than larvae isolated directly from the lungs of infected mammals. Using transmission electron microscopy, we demonstrated that there was little difference in the ultrastructure of Schistosoma japonicum schistosomula obtained by the two methods. However, significant differences in gene expression profiles were apparent when we used an oligonucleotide microarray to compare the gene expression profiles of schistosomula obtained in vivo from lung tissue with those maintained in vitro, and with adult worms of S. japonicum. It is likely that host environmental factors, which cannot be reliably reproduced in vitro, do influence the growth, development and overall biology of schistosomes. Thus caution is urged when using in vitro-cultured schistosomes and mechanically transformed/cultured schistosomula in molecular, biochemical and immunological studies.
Keyword Microarray
Schistosoma japonicum
Schistosomula
Gene expression
Culture conditions
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: ERA 2012 Admin Only
School of Medicine Publications
 
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