A contributory role for activated hepatic stellate cells in the dynamics of Schistosoma japonicum egg-induced fibrosis

Bartley, Paul B., Ramm, Grant A., Jones, Malcolm K., Ruddell, Richard G., Li, Yuesheng and McManus, Donald P. (2006) A contributory role for activated hepatic stellate cells in the dynamics of Schistosoma japonicum egg-induced fibrosis. International Journal for Parasitology, 36 9: 993-1001. doi:10.1016/j.ijpara.2006.04.015


Author Bartley, Paul B.
Ramm, Grant A.
Jones, Malcolm K.
Ruddell, Richard G.
Li, Yuesheng
McManus, Donald P.
Title A contributory role for activated hepatic stellate cells in the dynamics of Schistosoma japonicum egg-induced fibrosis
Formatted title
A contributory role for activated hepatic stellate cells in the dynamics of Schistosoma japonicum egg-induced fibrosis
Journal name International Journal for Parasitology   Check publisher's open access policy
ISSN 0020-7519
1879-0135
Publication date 2006-08
Sub-type Article (original research)
DOI 10.1016/j.ijpara.2006.04.015
Volume 36
Issue 9
Start page 993
End page 1001
Total pages 9
Place of publication Oxford, United Kingdom
Publisher Elsevier
Language eng
Formatted abstract
The disease manifestations of schistosomiasis arise from the mammalian host-mediated type 2 T-helper cell-induced (Th2) fibro-granulomatous inflammatory response to eggs trapped within host tissues. Activated hepatic stellate cells are well described as the effector cells of hepatic fibrosis in a variety of human diseases and rodent models. The aim of this study was to further understand the mechanism of fibrosis and the role of hepatic stellate cells in hepatic schistosomiasis progression. Groups of female CBA mice, which produce an intermediate degree of Schistosoma japonicum-induced liver fibrosis, were infected with S. japonicum, perfused at fortnightly time points and the liver tissue and contained egg granulomas examined by immunohistochemistry and cytokine and chemokine analysis using quantitative PCR. Immunohistochemistry demonstrated the presence of activated hepatic stellate cells in the periphery of egg granulomas, adjacent to fibrotic areas. Time course analysis demonstrated that the transcription of smooth muscle actin-α type 1 collagen, IL-4, IL-13, IL-13Rα2 and tissue inhibitor of metalloproteinase-1 mirrored the initial increase and subsequent down-modulation of granuloma diameter in mice. However, the transcription of monocyte chemo-attractant protein-1, Regulated upon Activation Normal T Cell Expressed and Secreted (RANTES), TNF-α, IFN-γ and matrix metalloproteinase-9 paralleled the evolution of the total liver disease burden. Transforming growth factor-β1 transcription did not appear to be of biological significance in this mouse model. Immunohistochemical analysis of human hepatic granulomas showed close association of smooth muscle actin-α-expressing cells with fibrosis in five available cases of end-stage (advanced) schistosomiasis japonica. We conclude that activated hepatic stellate cells play a contributory role in the granulomatous, fibrotic process induced by S. japonicum eggs, both in the murine model and in human disease.
Keyword Schistosome
Schistosomiasis
Activated hepatic stellate cell
Hepatic fibrosis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 55 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 65 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 05 Sep 2011, 18:03:38 EST by System User on behalf of School of Geography, Planning & Env Management