The promotion of neural progenitor cells proliferation by aligned and randomly oriented collagen nanofibers through beta 1 integrin/MAPK signaling pathway

Wang, Yansong, Yao, Meng, Zhou, Jihui, Zheng, Wei, Zhou, Changwei, Dong, Daming, Liu, Yugang, Teng, Zhaowei, Jiang, Yongqing, Wei, Guojen and Cui, Xiaoying (2011) The promotion of neural progenitor cells proliferation by aligned and randomly oriented collagen nanofibers through beta 1 integrin/MAPK signaling pathway. Biomaterials, 32 28: 6737-6744. doi:10.1016/j.biomaterials.2011.05.075


Author Wang, Yansong
Yao, Meng
Zhou, Jihui
Zheng, Wei
Zhou, Changwei
Dong, Daming
Liu, Yugang
Teng, Zhaowei
Jiang, Yongqing
Wei, Guojen
Cui, Xiaoying
Title The promotion of neural progenitor cells proliferation by aligned and randomly oriented collagen nanofibers through beta 1 integrin/MAPK signaling pathway
Journal name Biomaterials   Check publisher's open access policy
ISSN 0142-9612
1878-5905
Publication date 2011-10
Sub-type Article (original research)
DOI 10.1016/j.biomaterials.2011.05.075
Volume 32
Issue 28
Start page 6737
End page 6744
Total pages 8
Place of publication Amsterdam , The Netherlands
Publisher Elsevier
Collection year 2012
Language eng
Formatted abstract
In regenerative medicine, accumulating evidence demonstrates that the property of substrates monitors neural stem cells behavior. However, how stem cells sense and interpret biochemical and topographical cues remains elusive. This study aimed to explore the mechanism how nanofibrous scaffold modulated stem cells behavior. Spinal cord derived neural progenitor cells (NPCs) were cultured on electrospun aligned and randomly oriented collagen nanofibrous scaffolds. A 30% increase in proliferation and an elevation of BrdU incorporation were observed in NPCs on collagen nanofibers, compared to that on collagen-coated surface. In particular, NPCs expanded faster on aligned nanofibers in comparison with that on randomly oriented nanofibers. Moreover, an alteration in cell cycle progression with a reduced percentage of cells in G0/G1 phase and increased cell proliferation index (S phase plus G2/M phase) was also detected in NPCs cultured on collagen nanofibers. Incubating NPCs with anti-β1 integrin antibody or U1026 (an inhibitor of mitogen- activated protein kinase kinase, MEK) eliminated the altered cell cycle dynamics and BrdU incorporation induced by collagen nanofibers. In addition, cyclin D1 and cyclin dependent kinase 2 (CDK2), downstream genes of β1 integrin/mitogen-activated protein kinase (MAPK) pathway that control G1/S phase transition, were correspondingly regulated by nanofibers. Collectively, these data suggested that the property of substrate modulated NPCs proliferation by promoting cell cycle through β1 integrin/MAPK pathway. Our findings provide a better understanding of the interaction between NPCs and the substrate and therefore will pave way for regenerative medicine.
Keyword Collagen
Nanofibers
Neural progenitor
Proliferation
Cell cycle
ß1 integrin/MAPK
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
Official 2012 Collection
 
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