Bioenergy feedstock-specific enrichment of microbial populations during high-solids thermophilic deconstruction

Reddy, Amitha P., Allgaier, Martin, Singer, Steven W., Hazen, Terry C., Simmons, Blake A., Hugenholtz, Philip and VanderGheynst, Jean S. (2011) Bioenergy feedstock-specific enrichment of microbial populations during high-solids thermophilic deconstruction. Biotechnology and Bioengineering, 108 9: 2088-2098. doi:10.1002/bit.23176


Author Reddy, Amitha P.
Allgaier, Martin
Singer, Steven W.
Hazen, Terry C.
Simmons, Blake A.
Hugenholtz, Philip
VanderGheynst, Jean S.
Title Bioenergy feedstock-specific enrichment of microbial populations during high-solids thermophilic deconstruction
Journal name Biotechnology and Bioengineering   Check publisher's open access policy
ISSN 0006-3592
1097-0290
Publication date 2011-09-01
Sub-type Article (original research)
DOI 10.1002/bit.23176
Volume 108
Issue 9
Start page 2088
End page 2098
Total pages 11
Place of publication Hoboken, NJ, U.S.A.
Publisher John Wiley & Sons
Collection year 2012
Language eng
Formatted abstract
Thermophilic microbial communities that are active in a high-solids environment offer great potential for the discovery of industrially relevant enzymes that efficiently deconstruct bioenergy feedstocks. In this study, finished green waste compost was used as an inoculum source to enrich microbial communities and associated enzymes that hydrolyze cellulose and hemicellulose during thermophilic high-solids fermentation of the bioenergy feedstocks switchgrass and corn stover. Methods involving the disruption of enzyme and plant cell wall polysaccharide interactions were developed to recover xylanase and endoglucanase activity from deconstructed solids. Xylanase and endoglucanase activity increased by more than a factor of 5, upon four successive enrichments on switchgrass. Overall, the changes for switchgrass were more pronounced than for corn stover; solids reduction between the first and second enrichments increased by a factor of four for switchgrass while solids reduction remained relatively constant for corn stover. Amplicon pyrosequencing analysis of small-subunit ribosomal RNA genes recovered from enriched samples indicated rapid changes in the microbial communities between the first and second enrichment with the simplified communities achieved by the third enrichment. The results demonstrate a successful approach for enrichment of unique microbial communities and enzymes active in a thermophilic high-solids environment.
Keyword Switchgrass
Corn stover
Solid state fermentation
Compost
Xylanase
Endoglucanase
Cellulosic ethanol
Trichoderma-reesei
Pretreatment
Cellulases
Xylanase
Soil
Fermentation
Inhibition
Hydrolysis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Chemistry and Molecular Biosciences
 
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