Immunohistochemical examination of 5 novel biomarkers in oral epithelial dysplasia and squamous cell carcinoma

Abdulmajeed, A., Chan, A., Stein, S. and Farah, C. S. (2011). Immunohistochemical examination of 5 novel biomarkers in oral epithelial dysplasia and squamous cell carcinoma. In: Third World Congress of the International Academy of Oral Oncology 2011. 3rd World Congress of the International Academy of Oral Oncology, Singapore, Singapore, (S109-S110). 14-17 July 2011. doi:10.1016/j.oraloncology.2011.06.355


Author Abdulmajeed, A.
Chan, A.
Stein, S.
Farah, C. S.
Title of paper Immunohistochemical examination of 5 novel biomarkers in oral epithelial dysplasia and squamous cell carcinoma
Conference name 3rd World Congress of the International Academy of Oral Oncology
Conference location Singapore, Singapore
Conference dates 14-17 July 2011
Proceedings title Third World Congress of the International Academy of Oral Oncology 2011   Check publisher's open access policy
Journal name Oral Oncology   Check publisher's open access policy
Place of Publication Oxford, United Kingdom
Publisher Pergamon
Publication Year 2011
Sub-type Published abstract
DOI 10.1016/j.oraloncology.2011.06.355
ISSN 1368-8375
1744-7895
Volume 47
Issue Supp. 1
Start page S109
End page S110
Total pages 2
Collection year 2012
Language eng
Formatted Abstract/Summary
Introduction: In order to further investigate novel candidate genes for oral epithelial dysplasia (OED) classification and prognostication, we have reviewed microarray studies which analysed potentially malignant oral lesions and identified a set of commonly dysregulated genes and selected 5 genes for further analysis. Here we investigate the immunohistochemical expression pattern of CLSP, ELF3, IFI44, USP18, and CXCL13 in normal oral mucosa (NOR), OED and oral squamous cell carcinoma (OSCC) to determine if their expression could predict of the presence and degree of OED.

Methods:
A standard immunohistochemical technique was performed on 18 NOR, 27 mild dysplasia (MD), 13 moderate to severe dysplasia (SD), and 49 OSCC specimens.

Results: In this study, a gradual decrease of CLSP and ELF3 expression was found from NOR and MD to SD and OSCC. We found a significant decrease of CLSP expression in SD and OSCC compared to MD (p < 0.05) and a significant reduction of ELF3 expression in OSCC compared to the other groups (p < 0.05). We also noted a gradual increase in staining intensity for USP18 and IFI44 from NOR to MD to SD with peak expression in OSCC. However, no significant difference regarding USP18 expression level was found between the histopathologic groups. The level of IFI44 expression was significantly higher in SD and OSCC compared to NOR and MD (p < 0.05). No significant difference between histopathologic groups was found regarding CXCL13 expression.

Discussion: The gradual decrease of CLSP and ELF3 expression may be explained by the fact that CLSP and ELF3 have a role in late keratinocyte differentiation and it is expected to see a lesser degree of differentiation in OSCC. IFI44 which was found to be over-expressed in SD and OSCC in this study has been linked to metastasis and lymph node involvement in breast and colon cancer in other studies. In conclusion, a significant difference in expression levels of CLSP, ELF3 and IFI44 between MD and SD and OSCC was found in this study indicating that these genes could serve as objective markers for the presence of moderate to severe OED, and should be investigated further as markers for cancer progression.
Q-Index Code EX
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 16 July 2011

Document type: Conference Paper
Collections: Non HERDC
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