Development of an in-line HPLC fingerprint ion-trap mass spectrometric method for identification and quality control of Radix Scrophulariae

Jing, Jing, Chan, Chi-on, Xu, Lijia, Jin, Dengping, Cao, Xinwei, Mok, Daniel K. W., Parekh, H. S. and Chen, Sibao (2011) Development of an in-line HPLC fingerprint ion-trap mass spectrometric method for identification and quality control of Radix Scrophulariae. Journal of Pharmaceutical and Biomedical Analysis, 56 4: 830-835. doi:10.1016/j.jpba.2011.07.032

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Author Jing, Jing
Chan, Chi-on
Xu, Lijia
Jin, Dengping
Cao, Xinwei
Mok, Daniel K. W.
Parekh, H. S.
Chen, Sibao
Title Development of an in-line HPLC fingerprint ion-trap mass spectrometric method for identification and quality control of Radix Scrophulariae
Formatted title
Development of an in-line HPLC fingerprint ion-trap mass spectrometric method for identification and quality control of Radix Scrophulariae
Journal name Journal of Pharmaceutical and Biomedical Analysis   Check publisher's open access policy
ISSN 0731-7085
1873-264X
Publication date 2011-12-05
Sub-type Article (original research)
DOI 10.1016/j.jpba.2011.07.032
Volume 56
Issue 4
Start page 830
End page 835
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Collection year 2012
Language eng
Formatted abstract
Chromatographic fingerprinting has been widely accepted as a crucial method for qualitative and quantitative analyses of bioactives within traditional Chinese medicine. A fingerprint provides detailed information, specific for any given herb, thus facilitating the quality control measures of a given traditional Chinese medicine. In this article, quality assessment of Radix Scrophulariae was achieved by using high performance liquid chromatography combining diode-array detection and electrospray ionization mass spectrometry (HPLC–DAD–ESI/MS). Eight batches of sample obtained from different origins in China were used to establish the fingerprint and quantitative analyses. By comparing the retention times, UV and MS spectral data with reference standards, four characteristic peaks in the chromatograms were confirmed as corresponding to acetoside, angoroside C, cinnamic acid, and harpagoside. In addition, other two characteristic peaks were tentatively identified, following the literature interpretation of HPLC–ESI-MS and LC–MS/MS (affording structural information) to be sibirioside A and scrophuloside B4, respectively. The results indicated that the newly developed HPLC–DAD–MS fingerprint method would be suitable for quality control of Radix Scrophulariae.
Keyword High-performance liquid chromatography
Mass spectrometry
Fingerprint
Radix Scrophulariae
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published under Short Communications

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Pharmacy Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 16 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 26 Aug 2011, 15:36:09 EST by Charna Kovacevic on behalf of School of Pharmacy