Assessment of inhibitory potency of antibiotics by MRI : Apparent T-2 as a marker of cell growth

Hoerr, Verena, Hoffmann, Kerstin, Schollmayer, Curd, Holzgrabe, Ulrike, Haase, Axel, Jakob, Peter and Faber, Cornelius (2006) Assessment of inhibitory potency of antibiotics by MRI : Apparent T-2 as a marker of cell growth. Magnetic Resonance Materials in Physics Biology and Medicine, 19 5: 247-255. doi:10.1007/s10334-006-0053-z


Author Hoerr, Verena
Hoffmann, Kerstin
Schollmayer, Curd
Holzgrabe, Ulrike
Haase, Axel
Jakob, Peter
Faber, Cornelius
Title Assessment of inhibitory potency of antibiotics by MRI : Apparent T-2 as a marker of cell growth
Journal name Magnetic Resonance Materials in Physics Biology and Medicine   Check publisher's open access policy
ISSN 0968-5243
1352-8661
Publication date 2006-11
Sub-type Article (original research)
DOI 10.1007/s10334-006-0053-z
Volume 19
Issue 5
Start page 247
End page 255
Total pages 9
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Abstract A new method to assess the antibiotic potency by MRI has been developed. Correlating 1H NMR spectra of bacterial cultures with the extracellular parameters T2, OD600, and pH, a relationship between cell growth and T2 variations was established. T2 is influenced by chemical exchange that depends on pH, composition, and concentration of the medium. Changes in the medium from bacterial metabolism are reflected in alternating T2 values. At 17.6 T, growth curves based on T2 values were measured simultaneously of several cultures of Streptococcus vestibularis. From T2 growth curves in the presence of varying concentrations of vancomycin, the minimum inhibitory concentration of the antibiotic could be determined to be 0.33+/-0.08 microM. This value was in good agreement with the result obtained by the conventional broth microdilution. In principle, T2 growth curves can be determined on a large number of cultures simultaneously and may potentially be used as a novel tool in high through-put screening of novel anti-infective substances.
Keyword Chemical exchange
Antibiotics
Drug screening
Bacterial cultures
Metabolomics
Magnetic-Resonance Spectroscopy
Brain Abscesses
In-Vivo
Chemical-Exchange
Staphylococcus-Aureus
Aqueous-Solution
Amino-Acids
Identification
Nmr
Differentiation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: ERA 2012 Admin Only
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