A putative role for endogenous FGF-2 in FGF-1 mediated differentiation of human preadipocytes

Hutley, Louise J., Newell, Felicity S., Kim, Yu-Hee, Luo, Xiao, Widberg, Charlotte H., Shurety, Wenda, Prins, Johannes B. and Whitehead, Jonathan P. (2011) A putative role for endogenous FGF-2 in FGF-1 mediated differentiation of human preadipocytes. Molecular and Cellular Endocrinology, 339 1-2: 165-171. doi:10.1016/j.mce.2011.04.012

Author Hutley, Louise J.
Newell, Felicity S.
Kim, Yu-Hee
Luo, Xiao
Widberg, Charlotte H.
Shurety, Wenda
Prins, Johannes B.
Whitehead, Jonathan P.
Title A putative role for endogenous FGF-2 in FGF-1 mediated differentiation of human preadipocytes
Journal name Molecular and Cellular Endocrinology   Check publisher's open access policy
ISSN 0303-7207
Publication date 2011-06
Sub-type Article (original research)
DOI 10.1016/j.mce.2011.04.012
Volume 339
Issue 1-2
Start page 165
End page 171
Total pages 7
Place of publication Shannon, Co. Clare, Ireland
Publisher Elsevier Ireland
Collection year 2012
Language eng
Abstract The defining characteristic of obesity is increased adipose tissue (AT) mass following chronic positive energy supply. AT mass is determined by adipocyte number and size, which reflect proliferation and differentiation of preadipocytes and hypertrophy of pre-existing adipocytes. The molecular pathways governing AT expansion are incompletely defined. We previously reported that FGF-1 primes proliferating primary human preadipocytes (phPA), thereby increasing adipogenesis. Here we examined whether FGF-1's adipogenic actions were due to modulation of other FGFs. Treatment of phPA with FGF-1 reduced FGF-2 mRNA/protein by 80%. To examine a putative functional role we performed siRNA knockdown studies. Following FGF-2 knockdown preadipocyte proliferation was decreased and expression of adipogenic genes (PPARγ, G3PDH and adiponectin) was increased at day 1 of differentiation. These results suggest that changes in endogenous FGF-2 levels contribute to FGF-1's early adipogenic effects and highlight the complexity of the paracrine interplay between FGFs within human AT.
Keyword Fibroblast growth factor
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
UQ Diamantina Institute Publications
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