DNA amplification approaches for the diagnosis of key parasitic helminth infections of humans

Gordon, Catherine A., Gray, Darren J., Gobert, Geoffrey N. and McManus, Donald P. (2011) DNA amplification approaches for the diagnosis of key parasitic helminth infections of humans. Molecular and Cellular Probes, 25 4: 143-152. doi:10.1016/j.mcp.2011.05.002

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Author Gordon, Catherine A.
Gray, Darren J.
Gobert, Geoffrey N.
McManus, Donald P.
Title DNA amplification approaches for the diagnosis of key parasitic helminth infections of humans
Journal name Molecular and Cellular Probes   Check publisher's open access policy
ISSN 0890-8508
Publication date 2011-08
Sub-type Article (original research)
DOI 10.1016/j.mcp.2011.05.002
Volume 25
Issue 4
Start page 143
End page 152
Total pages 10
Place of publication Camden, London, U.K.
Publisher Academic Press
Collection year 2012
Language eng
Formatted abstract
Parasitic helminths of humans are a major public health threat, particularly in developing countries. The rapid and reliable diagnosis of helminth infections is central to their control as well as for environmental monitoring and disease surveillance. Effective diagnosis is dependent on the sensitivity, specificity and reliability of the tests employed. The most commonly used diagnosis of helminth infections has been the microscopic detection of eggs in faecal samples. However, the eggs of related helminths are often very similar in morphology and morphometry, making diagnosis inaccurate. DNA amplification tests can provide alternative approaches for diagnosis and can be sensitive and specific, provided reliable molecular genetic markers are employed in the tests. Such advanced tools can be used for the quantification of egg numbers, in order to assess infection intensities in infected hosts, and can provide multiplexing capability which can be required for the identification of multiple different helminth species in individual samples. These approaches provide powerful diagnostic techniques that can be used in clinical settings and as laboratory tools for surveillance and for environmental monitoring to support helminth control programs.
Keyword Parasitic helminths
Molecular diagnosis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Public Health Publications
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Citation counts: TR Web of Science Citation Count  Cited 17 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 19 times in Scopus Article | Citations
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