Slow-growing heterokaryons as potential intermediates in supernumerary chromosome transfer between biotypes of Colletotrichum gloeosporioides

Manners, John M. and He, Chaozu (2011) Slow-growing heterokaryons as potential intermediates in supernumerary chromosome transfer between biotypes of Colletotrichum gloeosporioides. Mycological Progress, 10 3: 383-388. doi:10.1007/s11557-011-0749-y


Author Manners, John M.
He, Chaozu
Title Slow-growing heterokaryons as potential intermediates in supernumerary chromosome transfer between biotypes of Colletotrichum gloeosporioides
Journal name Mycological Progress   Check publisher's open access policy
ISSN 1617-416X
1861-8952
Publication date 2011-08
Sub-type Article (original research)
DOI 10.1007/s11557-011-0749-y
Volume 10
Issue 3
Start page 383
End page 388
Total pages 6
Place of publication Heidelberg, Germany
Publisher Springer
Collection year 2012
Language eng
Formatted abstract
The vegetative transfer of a supernumerary chromosome between two biotypes (A and B) of Colletotrichum gloeosporioides pathogenic on Stylosanthes spp. has been previously demonstrated. The mechanism of transfer is unknown, and transient heterokaryosis has been implicated as an intermediary step, but inter-biotype heterokaryons have not so far been isolated. Conidia of a hygromycin-resistant strain of biotype A and a phleomycin-resistant strain of biotype B were mixed, co-cultured and plated on media containing both antibiotics and extremely slow-growing colonies resistant to both antibiotics isolated using repeated hyphal tip sub-culturing. Mononucleate conidia derived from these colonies were unable to germinate on media containing both antibiotics, but were able to germinate on media containing only one antibiotic, with hygromycin-resistant colonies predominating, indicating that unbalanced heterokaryons had formed. The heterokaryons had highly impaired growth rates suggesting some incompatibility. DNA marker analysis confirmed their heterokaryon status. These results demonstrate that unfit inter-biotype heterokaryons can form and potentially provide an intermediate step for supernumerary chromosomal exchange.
Keyword Vegetative compatibility
Parasexual cycle
Dispensable chromosome
Anastamosis
Horizontal gene transfer
Horizontal gene-transfer
Vegetatively incompatible biotypes
Pathogen nectria-haematococca
Conidial anastomosis tubes
Filamentous fungi
Stylosanthes-guianensis
Fusion
Pea
Expression
Hypothesis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Official 2012 Collection
 
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