Two phenylalanine ammonia-lyase genes (DcPAL2 and DcPAL3) are involved in the cut-induced stem-end wound reaction of carnation (Dianthus caryophyllus L.)

Zhang, Jidong, Cao, Jinping, Qiao, Aiming, Li, Hongmei, He, Shenggen, Wang, Weiquan, Sun, Min and Joyce, Daryl C. (2011) Two phenylalanine ammonia-lyase genes (DcPAL2 and DcPAL3) are involved in the cut-induced stem-end wound reaction of carnation (Dianthus caryophyllus L.). Journal of Horticultural Science and Biotechnology, 86 4: 377-383.

Author Zhang, Jidong
Cao, Jinping
Qiao, Aiming
Li, Hongmei
He, Shenggen
Wang, Weiquan
Sun, Min
Joyce, Daryl C.
Title Two phenylalanine ammonia-lyase genes (DcPAL2 and DcPAL3) are involved in the cut-induced stem-end wound reaction of carnation (Dianthus caryophyllus L.)
Journal name Journal of Horticultural Science and Biotechnology   Check publisher's open access policy
ISSN 1462-0316
Publication date 2011-07
Sub-type Article (original research)
Volume 86
Issue 4
Start page 377
End page 383
Total pages 7
Place of publication Kent, England, U.K.
Publisher Headley Brothers
Collection year 2012
Language eng
Formatted abstract
The cut stem-end wound reaction is potentially important during the physical blockage of water uptake in cut flowers. Phenylalanine ammonia-lyase (PAL; EC 4.3.1.24) is involved in plant wound reactions, being the key regulatory enzyme of phenylpropanoid metabolism. Two full-length PAL genes (DcPAL2 and DcPAL3) cDNAs were isolated from cut carnation (Dianthus caryophyllus L.) flower stem-ends and found to be transiently expressed following wounding. DcPAL2 was 2,439 bp-long with an open reading frame (ORF) encoding a putative protein of 718 amino acids. DcPAL3 was 2,453 bp-long with an ORF encoding a putative protein of 709 amino acids. An increase in PAL enzyme activity within the basal 2-cm stem-end of cut carnation flowers commenced 12 h after wounding and peaked at 48 h. However, DcPAL2 and DcPAL3 transcript levels increased earlier than PAL activity. DcPAL2 transcript levels increased gradually to a peak 12 h after wounding and remained relatively high thereafter. In contrast, DcPAL3 gene transcripts peaked 3 h after wounding, then decreased. A third PAL gene, DcPAL1, showed constitutively high levels of expression, with only a slight change after wounding. Collectively, these results suggest that expression of DcPAL2 and DcPAL3 leads to increased PAL activity during the wound reaction in cut carnation stems.
Keyword Induced Xylem Occlusion
Chrysanthemum Flowers
Exogenous Ethylene
Methyl Jasmonate
Fresh-Cut
Expression
Lettuce
Quality
Fruit
Accumulation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Agriculture and Food Sciences
 
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