Transcription and expression of plasmodium falciparum histidine-rich proteins in different stages and strains: implications for rapid diagnostic tests

Baker, Joanne, Gatton, Michelle L., Peters, Jennifer, Ho, Mei-Fong, McCarthy, James S. and Cheng, Qin (2011) Transcription and expression of plasmodium falciparum histidine-rich proteins in different stages and strains: implications for rapid diagnostic tests. Plos One, 6 7: -10. doi:10.1371/journal.pone.0022593


Author Baker, Joanne
Gatton, Michelle L.
Peters, Jennifer
Ho, Mei-Fong
McCarthy, James S.
Cheng, Qin
Title Transcription and expression of plasmodium falciparum histidine-rich proteins in different stages and strains: implications for rapid diagnostic tests
Formatted title
Transcription and expression of plasmodium falciparum histidine-rich proteins in different stages and strains: Implications for rapid diagnostic tests
Journal name Plos One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2011-07-01
Sub-type Article (original research)
DOI 10.1371/journal.pone.0022593
Open Access Status DOI
Volume 6
Issue 7
End page 10
Total pages 10
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2012
Language eng
Formatted abstract
Background: Although rapid diagnostic tests (RDTs) for Plasmodium falciparum infection that target histidine rich protein 2 (PfHRP2) are generally sensitive, their performance has been reported to be variable. One possible explanation for variable test performance is differences in expression level of PfHRP in different parasite isolates.

Methods: Total RNA and protein were extracted from synchronised cultures of 7 P. falciparum lines over 5 time points of the life cycle, and from synchronised ring stages of 10 falciparum lines. Using quantitative real-time polymerase chain reaction, Western blot analysis and ELISA we investigated variations in the transcription and protein levels of pfhrp2, pfhrp3 and PfHRP respectively in the different parasite lines, over the parasite intraerythrocytic life cycle.

Results: Transcription of pfhrp2 and pfhrp3 in different parasite lines over the parasite life cycle was observed to vary relative to the control parasite K1. In some parasite lines very low transcription of these genes was observed. The peak transcription was observed in ring-stage parasites. Pfhrp2 transcription was observed to be consistently higher than pfhrp3 transcription within parasite lines. The intraerythrocytic lifecycle stage at which the peak level of protein was present varied across strains. Total protein levels were more constant relative to total mRNA transcription, however a maximum 24 fold difference in expression at ring-stage parasites relative to the K1 strain was observed.

Conclusions: The levels of transcription of pfhrp2 and pfhrp3, and protein expression of PfHRP varied between different P. falciparum strains. This variation may impact on the detection sensitivity of PfHRP2-detecting RDTs.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Public Health Publications
School of Medicine Publications
 
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