Characterization of mesophilic bacilli in faeces of feedlot cattle

Wu, X. -Y., Walker, M., Vanselow, B., Chao, R. -L. and Chin, J. (2007) Characterization of mesophilic bacilli in faeces of feedlot cattle. Journal of Applied Microbiology, 102 3: 872-879. doi:10.1111/j.1365-2672.2006.03106.x

Author Wu, X. -Y.
Walker, M.
Vanselow, B.
Chao, R. -L.
Chin, J.
Title Characterization of mesophilic bacilli in faeces of feedlot cattle
Journal name Journal of Applied Microbiology   Check publisher's open access policy
ISSN 1364-5072
Publication date 2007-03
Sub-type Article (original research)
DOI 10.1111/j.1365-2672.2006.03106.x
Volume 102
Issue 3
Start page 872
End page 879
Total pages 8
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Aims:  To determine the identity and composition of mesophilic Bacillus spp. in faeces sampled from feedlot cattle.
Methods and Results:  Faecal samples from 10 feedlot cattle were analysed. The total aerobic spore count increased from 4·6 × 104 CFU g−1 (before feedlotting, day 0) to 1·6 × 106 CFU g−1 (feedlot for day 76). A total of 150 randomly selected spore isolates (60 each from days 0 and 76 cattle, 30 from feed) were speciated using a Bacillus group-specific PCR-amplified ribosomal DNA restriction analysis technique (Wu et al. 2006). At day 0, Bacillus subtilis and Bacillus cereus predominated with a prevalence of 58·3% and 26·7%, respectively, whereas three species, B. subtilis (50·0%), Bacillus licheniformis (27·6%) and Bacillus clausii (20·0%) predominated in day 76 faecal samples. Of these, only the first two species were present in feed samples at a frequency of 70% and 30% respectively. All B. cereus isolates on day 0, possessed at least one of three enterotoxin genes (nheA, nheB and nheC) but these were completely eliminated after a period of feedlotting. All isolates of B. licheniformis were genotypically heterogeneous according to pulsed-field gel electrophoresis analysis.
Conclusions:  Cattle faeces contain large numbers of Bacillus spores representing different mesophilic species. Stable faecal populations of particular Bacillus spp. mimicking those found in feed, were subsequently established by feedlotting.
Significance and Impact of the Study:  The results obtained and methods used in this study will help to investigate the indigenous Bacillus composition in the gastrointestinal tract of cattle and will further guide the administration of Bacillus probiotics.
Keyword Amplified ribosomal DNA restriction analysis
Bacillus identification
Cattle faecal sample
Pulsed-field gel electrophoresis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
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