Antigen-Specific T-Cell Responses to a Recombinant Fowlpox Virus Are Dependent on MyD88 and Interleukin-18 and Independent of Toll-Like Receptor 7 (TLR7)- and TLR9-Mediated Innate Immune Recognition

Lousberg, Erin L., Diener, Kerrilyn R., Fraser, Cara K., Phipps, Simon, Foster, Paul S., Chen, Weisan, Uematsu, Satoshi, Akira, Shizuo, Robertson, Sarah A., Brown, Michael P. and Hayball, John D. (2011) Antigen-Specific T-Cell Responses to a Recombinant Fowlpox Virus Are Dependent on MyD88 and Interleukin-18 and Independent of Toll-Like Receptor 7 (TLR7)- and TLR9-Mediated Innate Immune Recognition. Journal of Virology, 85 7: 3385-3396.


Author Lousberg, Erin L.
Diener, Kerrilyn R.
Fraser, Cara K.
Phipps, Simon
Foster, Paul S.
Chen, Weisan
Uematsu, Satoshi
Akira, Shizuo
Robertson, Sarah A.
Brown, Michael P.
Hayball, John D.
Title Antigen-Specific T-Cell Responses to a Recombinant Fowlpox Virus Are Dependent on MyD88 and Interleukin-18 and Independent of Toll-Like Receptor 7 (TLR7)- and TLR9-Mediated Innate Immune Recognition
Journal name Journal of Virology  (ERA 2012 Listed)    (ERA 2010 Rank A*)   Check publisher's open access policy
Publication date 2011-04
Sub-type Article
DOI 10.1128/JVI.02000-10
Volume number 85
Issue number 7
ISSN 0022-538X
1098-5514
Start page 3385
End page 3396
Total pages 12
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2012
Language eng
Formatted abstract Fowlpox virus (FWPV) is a double-stranded DNA virus long used as a live-attenuated vaccine against poultry diseases, but more recent interest has focused on its use as a mammalian vaccine vector. Here, in a mouse model system using FWPV encoding the nominal target antigen chicken ovalbumin (OVA) (FWPVOVA), we describe for the first time some of the fundamental processes by which FWPV engages both the innate and adaptive immune systems. We show that Toll-like receptor 7 (TLR7) and TLR9 are important for type I interferon secretion by dendritic cells, while TLR9 is solely required for proinflammatory cytokine secretion. Despite this functional role for TLR7 and TLR9 in vitro, only the adapter protein myeloid differentiation primary response gene 88 (MyD88) was shown to be essential for the formation of adaptive immunity to FWPVOVA in vivo. The dependence on MyD88 was confined only to the T-cell compartment and was not related to its contribution to TLR signaling, dendritic cell maturation, or the capture and presentation of FWPVderived OVA antigen. We demonstrate that this is not by means of mediating T-cell-dependent interleukin-1 (IL-1) signaling, but rather, we suggest that MyD88 functions to support T-cell-specific IL-18 receptor signaling, which in turn is essential for the formation of adaptive immunity to FWPV-encoded OVA.
Keyword Plasmacytoid dendritic cells
Gamma-binding protein
Bone marrow cultures
Double stranded RNA
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article
Collections: Official 2012 Collection
School of Biomedical Sciences Publications
 
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