Genetic changes in two cell lines from human malignant mesothelioma during long term culturing

Relan, V., Quinn, K. R., Wright, C. M., Savarimuthu, S. M., Tan, M. E., Clarke, B. E., Duhig, E. E., Yang, I. A., Fong, K. M. and Bowman, R. V. (2011). Genetic changes in two cell lines from human malignant mesothelioma during long term culturing. In: Supplement to Journal of Thoracic Oncology: 3rd Australian Lung Cancer Conference (ALCC). 3rd Australian Lung Cancer Conference (ALCC), Melbourne, Australia, (S33-S33). 6-9 October 2010.

Author Relan, V.
Quinn, K. R.
Wright, C. M.
Savarimuthu, S. M.
Tan, M. E.
Clarke, B. E.
Duhig, E. E.
Yang, I. A.
Fong, K. M.
Bowman, R. V.
Title of paper Genetic changes in two cell lines from human malignant mesothelioma during long term culturing
Conference name 3rd Australian Lung Cancer Conference (ALCC)
Conference location Melbourne, Australia
Conference dates 6-9 October 2010
Proceedings title Supplement to Journal of Thoracic Oncology: 3rd Australian Lung Cancer Conference (ALCC)   Check publisher's open access policy
Journal name Journal of Thoracic Oncology   Check publisher's open access policy
Place of Publication Philadelphia PA, United States
Publisher Lippincott Williams & Wilkins
Publication Year 2011
Sub-type Published abstract
Open Access Status
ISSN 1556-1380
1556-0864
Volume 6
Issue 3, Supp. 1
Start page S33
End page S33
Total pages 1
Collection year 2012
Language eng
Formatted Abstract/Summary
Aim: This study aimed to assess genetic changes during long term culturing of two malignant mesothelioma (MM) cell lines (MM04 and MM05).

Methods: Cell lines were established from fresh resected pleural tumour tissues. Morphological changes between early passage (<10) and late passage (>30) were analyzed using standard light microscopy and Transmission Electron Microscopy (TEM). Growth characteristics were determined from growth curves. Gene expression differences between early and late passages were determined using Illumina’s HumanHT-12_v3_BeadChip providing coverage of >20,000 genes. Cytogenetic
abnormalities were determined using Spectral karyotyping (SKY).

Results and Discussion: Spindle shaped cells were observed in both early and late passages of both cell lines. TEM showed similar ultra-structural features in early and late passages in MM05. Doubling times were also similar between early (P9, 46hours) and late passage (P47, 43 hours). T Test (P < 0.001) on gene expression profile displayed down regulation of 24 genes (TMEM165, GREB1, HS566426, PRUNE, SLC10A7, C4ORF38, TBN, USP53 etc) and up regulation of 18 genes (TMEM2, CDKAL1, TIAM2, RPL23A, STAT3, UPK1B etc) in later passages. Although morphology and growth of late passage cell lines are similar to early passages, gene expression and cytogenetetic alterations were observed possibly reflecting adaptation and seletion pressures in cell culture.
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Published under Poster Abstracts: 3rd Australian Lung Cancer Conference (ALCC).

Document type: Conference Paper
Collections: Temporary Review
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Created: Mon, 15 Aug 2011, 15:22:13 EST by Mr Stephen Woods on behalf of Medicine - Prince Charles Hospital