VAMP3 regulates podosome organisation in macrophages and together with Stx4/SNAP23 mediates adhesion, cell spreading and persistent migration

Veale, Kelly J., Offenhauser, Carolin, Lei, Nazi, Stanley, Amanda C., Stow, Jennifer L. and Murray, Rachael Z. (2011) VAMP3 regulates podosome organisation in macrophages and together with Stx4/SNAP23 mediates adhesion, cell spreading and persistent migration. Experimental Cell Research, 317 13: 1817-1829. doi:10.1016/j.yexcr.2011.04.016


Author Veale, Kelly J.
Offenhauser, Carolin
Lei, Nazi
Stanley, Amanda C.
Stow, Jennifer L.
Murray, Rachael Z.
Title VAMP3 regulates podosome organisation in macrophages and together with Stx4/SNAP23 mediates adhesion, cell spreading and persistent migration
Journal name Experimental Cell Research   Check publisher's open access policy
ISSN 0014-4827
1090-2422
Publication date 2011-08
Sub-type Article (original research)
DOI 10.1016/j.yexcr.2011.04.016
Volume 317
Issue 13
Start page 1817
End page 1829
Total pages 13
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Collection year 2012
Language eng
Abstract The ability of cells to adhere, spread and migrate is essential to many physiological processes, particularly in the immune system where cells must traffic to sites of inflammation and injury. By altering the levels of individual components of the VAMP3/Stx4/SNAP23 complex we show here that this SNARE complex regulates efficient macrophage adhesion, spreading and migration on fibronectin. During cell spreading this complex mediates the polarised exocytosis of VAMP3-positive recycling endosome membrane into areas of membrane expansion, where VAMP3's surface partner Q-SNARE complex Stx4/SNAP23 was found to accumulate. Lowering the levels of VAMP3 in spreading cells resulted in a more rounded cell morphology and most cells were found to be devoid of the typical ring-like podosome superstructures seen normally in spreading cells. In migrating cells lowering VAMP3 levels disrupted the polarised localisation of podosome clusters. The reduced trafficking of recycling endosome membrane to sites of cell spreading and the disorganised podosome localisation in migrating macrophages greatly reduced their ability to persistently migrate on fibronectin. Thus, this important SNARE complex facilitates macrophage adhesion, spreading, and persistent macrophage migration on fibronectin through the delivery of VAMP3-positive membrane with its cargo to expand the plasma membrane and to participate in organising adhesive podosome structures.
Keyword Snare
Integrin
Podosome
Membrane fusion
Recycling endosome
Migration
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Institute for Molecular Bioscience - Publications
 
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