Quantification of L-alanyl-L-glutamine in mammalian cell culture broth: Evaluation of different detectors

Kromer, Jens O., Dietmair, Stefanie, Jacob, Shana S. and Nielsen, Lars K. (2011) Quantification of L-alanyl-L-glutamine in mammalian cell culture broth: Evaluation of different detectors. Analytical Biochemistry, 416 1: 129-131. doi:10.1016/j.ab.2011.05.019


Author Kromer, Jens O.
Dietmair, Stefanie
Jacob, Shana S.
Nielsen, Lars K.
Title Quantification of L-alanyl-L-glutamine in mammalian cell culture broth: Evaluation of different detectors
Formatted title
Quantification of L-alanyl-L-glutamine in mammalian cell culture broth: Evaluation of different detectors
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 0003-2697
1096-0309
Publication date 2011-09-01
Sub-type Article (original research)
DOI 10.1016/j.ab.2011.05.019
Volume 416
Issue 1
Start page 129
End page 131
Total pages 3
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Collection year 2012
Language eng
Formatted abstract
L-Alanyl-L-glutamine (also known as Ala-Gln or GlutaMAX) is widely used as a stable L-glutamine source in cell culture for the production of biopharmaceuticals. System approaches for the optimization of production processes require the analysis of all major substrates and products. We have compared four alternative detection systems for L-alanyl-L-glutamine in culture broth. Matrix effects prevented the use of ultraviolet or evaporative light scattering detection. Fluorescence detection used in routine amino acid protocols is compatible with culture broth and has a broad linear dynamic range. Mass spectrometry has superior sensitivity and can be integrated into quantitative metabolomic workflows.
Keyword Kinetics
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published under "Notes & Tips"

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Sun, 14 Aug 2011, 00:35:58 EST by System User on behalf of Aust Institute for Bioengineering & Nanotechnology