Immunophenotype of skin lymphocytic infiltrate in patients co-infected with Mycobacterium leprae and human immunodeficiency virus: A scenario dependent on CD8+ and/or CD20+ cells

Massone, C., Talhari, C., Talhari, S., Brunasso, A. M. G., Campbell, T. M., Curcic, P., Cerroni, L. and Ribeiro-Rodrigues, R. (2011) Immunophenotype of skin lymphocytic infiltrate in patients co-infected with Mycobacterium leprae and human immunodeficiency virus: A scenario dependent on CD8+ and/or CD20+ cells. British Journal of Dermatology, 165 2: 321-328. doi:10.1111/j.1365-2133.2011.10412.x


Author Massone, C.
Talhari, C.
Talhari, S.
Brunasso, A. M. G.
Campbell, T. M.
Curcic, P.
Cerroni, L.
Ribeiro-Rodrigues, R.
Title Immunophenotype of skin lymphocytic infiltrate in patients co-infected with Mycobacterium leprae and human immunodeficiency virus: A scenario dependent on CD8+ and/or CD20+ cells
Formatted title
Immunophenotype of skin lymphocytic infiltrate in patients co-infected with Mycobacterium leprae and human immunodeficiency virus: A scenario dependent on CD8+ and/or CD20+ cells
Journal name British Journal of Dermatology   Check publisher's open access policy
ISSN 0007-0963
1365-2133
Publication date 2011-08
Sub-type Article (original research)
DOI 10.1111/j.1365-2133.2011.10412.x
Volume 165
Issue 2
Start page 321
End page 328
Total pages 8
Place of publication Oxford, U.K.
Publisher Wiley-Blackwell Publishing
Collection year 2012
Language eng
Formatted abstract
Background: Leprosy occurs rarely in human immunodeficiency virus (HIV)-positive patients. In contrast to tuberculosis, there has been no report to date of an increase in HIV prevalence among patients with leprosy or of differences in leprosy’s clinical spectrum. While several studies describe the systemic immune response profile in patients co-infected with HIV and leprosy, the local immune skin response has been evaluated in only a small number of case reports and limited series of patients.

Objective: To investigate the interaction between Mycobacterium leprae and HIV infection in the skin.

Methods: We investigated the presence and frequency of cells positive for CD4, CD8, CD20, TIA-1, FOXP3 and CD123 in lymphocytic infiltrates from 16 skin biopsies taken from 15 patients with HIV–leprosy co-infection.

Results: CD4+ cells were absent in infiltrates from 6 (38%) skin biopsies and present in 10 (62%) cases at low levels (< 1·16%) of the lymphocytic infiltrate. CD8+ was the predominant phenotype in the infiltrate (99·4%), followed by TIA-1, expressed by > 75% of CD8+ cells. FOXP3+ cells were also present, representing 3·4% of the lymphocytic infiltrate. CD20+ cells were detected in 75% of the cases; however, in two cases (12%) these cells represented 25–50% of the infiltrate, while in the other 10 cases (62%) they were present only focally (< 25% of the infiltrate). CD123+ cells were not observed in any of the studied specimens.

Conclusions: Data presented here suggest that cell-mediated immune responses to M. leprae are preserved at the site of disease and that in the absence of CD4+ cells, CD8+FOXP3+ and CD20+ cells may be involved in granuloma formation.
Keyword Reconstitution inflammatory syndrome
Regulatory T-cells
Human tuberculosis
Foxp3 expression
Brazilian cohort
Immune-response
HIV coinfection
B-cells
Leprosy
Lesions
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Medicine Publications
 
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