Effect of MC1R variant allele status on MSH-ligand induction of dopachrome tautomerase in melanocytes co-cultured with keratinocytes

Ainger, Stephen A., Wong, Shu S., Roberts, Donald W., Leonard, J. Helen and Sturm, Richard A. (2011) Effect of MC1R variant allele status on MSH-ligand induction of dopachrome tautomerase in melanocytes co-cultured with keratinocytes. Experimental Dermatology, 20 8: 681-684. doi:10.1111/j.1600-0625.2011.01293.x

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Author Ainger, Stephen A.
Wong, Shu S.
Roberts, Donald W.
Leonard, J. Helen
Sturm, Richard A.
Title Effect of MC1R variant allele status on MSH-ligand induction of dopachrome tautomerase in melanocytes co-cultured with keratinocytes
Journal name Experimental Dermatology   Check publisher's open access policy
ISSN 0906-6705
1600-0625
Publication date 2011-08
Sub-type Letter to editor, brief commentary or brief communication
DOI 10.1111/j.1600-0625.2011.01293.x
Volume 20
Issue 8
Start page 681
End page 684
Total pages 4
Place of publication Oxford, U.K.
Publisher Wiley-Blackwell Publishing
Collection year 2012
Language eng
Formatted abstract
A co-culture system of melanocytic cells and keratinocytes was used to examine dendricity and dopachrome tautomerase (DCT) responses in low penetrant ‘r’ homozygote and ‘R/+’ heterozygote MC1R variant allele expressing cells compared to that of wild-type (WT) cells. The V60L−/− homozygote r variant cells showed similar responses to ligand as WT MC1R strains, while V92M−/− homozygote r variant cells were generally shown to have greater dendricity and express higher DCT than the WT cells, even at basal levels. The R151C+/− heterozygote cells showed similar responses to WT cells, while the R160W+/− and D294H+/− variant cells were reduced in their responses to NDP-MSH, but still had an active cAMP response with forskolin treatment. These responses are consistent with the dominant negative effect of these alleles on the MC1R WT allele that has previously been demonstrated genetically and biochemically.
Keyword Dopachrome tautomerase
Melanocortin-1 receptor
NDP-MSH
Pigmentation
Skin-cancer risk
Oxidative stress
Red hair
Expression
Melanogenesis
Polymorphisms
Melanoma
Melanocortin-1-receptor
Pigmentation
Phenotypes
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Letter to editor, brief commentary or brief communication
Collections: Official 2012 Collection
School of Medicine Publications
Institute for Molecular Bioscience - Publications
 
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