Evidence of compositional differences between the extracellular and intracellular DNA of a granular sludge biofilm

Cheng, M., Cook, A. E., Fukushima, T. and Bond, P. L. (2011) Evidence of compositional differences between the extracellular and intracellular DNA of a granular sludge biofilm. Letters in Applied Microbiology, 53 1: 1-7. doi:10.1111/j.1472-765X.2011.03074.x


Author Cheng, M.
Cook, A. E.
Fukushima, T.
Bond, P. L.
Title Evidence of compositional differences between the extracellular and intracellular DNA of a granular sludge biofilm
Journal name Letters in Applied Microbiology   Check publisher's open access policy
ISSN 0266-8254
1472-765X
Publication date 2011-07
Sub-type Article (original research)
DOI 10.1111/j.1472-765X.2011.03074.x
Volume 53
Issue 1
Start page 1
End page 7
Total pages 7
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Collection year 2012
Language eng
Formatted abstract
Aims: Extracellular polymeric substances (EPS) are an important component of microbial biofilms, and it is becoming increasingly apparent that extracellular DNA (eDNA) has a functional role in EPS. This study characterizes the eDNA extracted from the novel activated sludge biofilm process of aerobic granules.

Methods and Results: Exposing the sludge to cation exchange resin (CER) was used for the extraction of eDNA and intracellular DNA (iDNA) from aerobic granules. This was optimized for eDNA yield while causing minimal cell lysis. We then compared the DNA composition of these extractions using randomly amplified polymorphic DNA (RAPD) fingerprinting and PCR-based denaturing gradient-gel electrophoresis (DGGE). Upon the analysis of the genomic DNA and the 16S rRNA genes, differences were detected between the sludge biofilm eDNA and iDNA.

Conclusions: Different bacteria within the biofilm disproportionally release DNA into the EPS matrix of the biofilm.

Significance and Impact of the Study: The findings further the idea that eDNA has a functional role in the biofilm state, which is an important conceptual information for industrial application of biofilms. 
Keyword Aerobic granular sludge
Biofilm
Cation exchange resin
Extracellular polymeric substances extraction
Extracellular DNA
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Advanced Water Management Centre Publications
 
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