Selective transport of a peptide from its mixture with an amino acid using a supported liquid membrane process

Hossain, Md. M. and Stanley, R. A. (1995) Selective transport of a peptide from its mixture with an amino acid using a supported liquid membrane process. Separation Science and Technology, 30 20: 3801-3819. doi:10.1080/01496399508015144


Author Hossain, Md. M.
Stanley, R. A.
Title Selective transport of a peptide from its mixture with an amino acid using a supported liquid membrane process
Journal name Separation Science and Technology   Check publisher's open access policy
ISSN 0149-6395
1520-5754
Publication date 1995
Sub-type Article (original research)
DOI 10.1080/01496399508015144
Volume 30
Issue 20
Start page 3801
End page 3819
Total pages 19
Place of publication Philadelphia, PA, United States
Publisher Taylor & Francis
Language eng
Abstract Some experimental results on the separation of a peptide from a mixture of peptide and amino acid through a continuous supported liquid membrane process are described. As a model system a mixture of tryptophan (Trp, an amino acid) and tryptophan-leucine (Trp-Leu, a dipeptide) was chosen. The liquid membrane contained an anionic surfactant, sodium di-2-ethylhexyl sulfosuccinate (Aerosol OT/AOT), as a carrier dissolved in oleyl alcohol, as an organic solvent, supported on a commercial grade support (Celgard 2500). The liquid-liquid extraction experiments were carried out to study the influence of feed pH, feed and strip flow rates, and feed and carrier concentrations on the selective transport of Trp-Leu from its mixture with Trp. At pH 4–5 the transport rate of Trp-Leu was significantly higher than that of Trp. The increase in flow rate up to a value of 40 mL/h did not effect the selective removal of Trp-Leu. The flux rate increased with an increase of the carrier concentration up to 20% AOT, but the selectivity for Trp-Leu was highest at 10% AOT. An increase of Trp concentration up to 10-fold had little effect on the flux rate of Trp-Leu. The stability of SLM system in continuous removal of Trp-Leu from a single component system and from a binary mixture with Trp was studied. The effect of a competitive component did not alter significantly the flux rate and long-time performance in continuous operation. A procedure for regenerating SLM was examined, and the regenerated SLM performed as good as the freshly prepared one.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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