Effects of flavonoid extract Enzogenol® with vitamin C on protein oxidation and DNA damage in older human subjects

Senthilmohan, Senti T., Zhang, Jingli and Stanley, Roger A. (2003) Effects of flavonoid extract Enzogenol® with vitamin C on protein oxidation and DNA damage in older human subjects. Nutrition Research, 23 9: 1199-1210.


Author Senthilmohan, Senti T.
Zhang, Jingli
Stanley, Roger A.
Title Effects of flavonoid extract Enzogenol® with vitamin C on protein oxidation and DNA damage in older human subjects
Journal name Nutrition Research   Check publisher's open access policy
ISSN 0271-5317
1879-0739
Publication date 2003-09
Sub-type Article (original research)
DOI 10.1016/S0271-5317(03)00127-1
Volume 23
Issue 9
Start page 1199
End page 1210
Total pages 12
Place of publication New York, NY, United States
Publisher Elsevier
Language eng
Formatted abstract In this study a 12 week clinical trial was conducted with Enzogenol®, a commercially available proanthocyanidin -rich flavonoid extract derived from the bark of Pinus radiata that was formulated with vitamin C. The study was to determine whether the oxidative injury markers of protein and DNA damage could be affected by the product. Twenty four (14 males and 10 females) subjects aged between 55-75 years completed the study. The group was given a twice daily dose of 240 mg of Enzogenol® and 120 mg vitamin C for 12 weeks and blood samples were collected at the start of the study before supplementation, 6 weeks and 12 weeks. Plasma samples were analysed for protein carbonyl concentrations as a measure of protein oxidation by an ELISA method. Isolated peripheral blood mononuclear cells were analysed for DNA damage using the alkaline comet assay. Protein carbonyl concentration reductions were highly significant after 6 and 12 weeks of supplementation. DNA damage reduction, as measured by the comet assay, was not significant after 6 weeks but highly significant after 12 weeks of supplementation.
Keyword Flavonoid
Protein oxidation
DNA damage
Pine bark
Proanthocyanidin
Antioxidant
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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