Determination of the persistence of tetracycline antibiotics and their degradates in manure-amended soil using enzyme-linked immunosorbent assay and liquid chromatography-mass spectrometry

Aga, Diana S., O'Connor, Seamus, Ensley, Steve, Payero, Jose O., Snow, Daniel and Tarkalson, David (2005) Determination of the persistence of tetracycline antibiotics and their degradates in manure-amended soil using enzyme-linked immunosorbent assay and liquid chromatography-mass spectrometry. Journal of Agricultural and Food Chemistry, 53 18: 7165-7171. doi:10.1021/jf050415


Author Aga, Diana S.
O'Connor, Seamus
Ensley, Steve
Payero, Jose O.
Snow, Daniel
Tarkalson, David
Title Determination of the persistence of tetracycline antibiotics and their degradates in manure-amended soil using enzyme-linked immunosorbent assay and liquid chromatography-mass spectrometry
Journal name Journal of Agricultural and Food Chemistry   Check publisher's open access policy
ISSN 0021-8561
1520-5118
Publication date 2005-09-07
Sub-type Article (original research)
DOI 10.1021/jf050415
Volume 53
Issue 18
Start page 7165
End page 7171
Total pages 7
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Abstract The persistence of manure-borne oxytetracycline in soil was investigated under field conditions. Soil cores were collected approximately once a month for over a period of two years and subsampled at depth increments of 0−5, 5−10, 10−15, 15−36, and 36−71 cm. Soil samples were analyzed by enzyme-linked immunosorbent assay (ELISA) and/or by liquid chromatography−mass spectrometry (LC-MS). Whereas LC-MS showed that oxytetracycline declined to <50% of its initial soil concentration after 3 weeks, ELISA showed that the total tetracyclines did not decline significantly 5 months after manure application. The differences between ELISA and LC-MS results are attributed to the broad cross-reactivity of the antibodies employed, which detect many structurally related tetracyclines, including their isomers and degradation products. Only trace amounts (≤1.0 μg/kg) of oxytetracycline were observed in the subsurface soil, and none was detected in water samples from field lysimeters, suggesting that oxytetracycline has low mobility in soil.
Keyword Antibiotics
Manure
Elisa
Lc-Ms
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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