Regulation of alternative polyadenylation by genomic imprinting

Wood, Andrew J., Schulz, Reiner, Woodfine, Kathryn, Koltowska, Katarzyna, Beechey, Colin V., Peters, Jo, Bourc'his, Deborah and Oakey, Rebecca J. (2008) Regulation of alternative polyadenylation by genomic imprinting. Genes and Development, 22 9: 1141-1146. doi:10.1101/gad.473408

Author Wood, Andrew J.
Schulz, Reiner
Woodfine, Kathryn
Koltowska, Katarzyna
Beechey, Colin V.
Peters, Jo
Bourc'his, Deborah
Oakey, Rebecca J.
Title Regulation of alternative polyadenylation by genomic imprinting
Journal name Genes and Development   Check publisher's open access policy
ISSN 0890-9369
Publication date 2008-05
Sub-type Article (original research)
DOI 10.1101/gad.473408
Open Access Status DOI
Volume 22
Issue 9
Start page 1141
End page 1146
Total pages 6
Place of publication Woodbury, NY, United States
Publisher Cold Spring Harbor Laboratory Press
Language eng
Formatted abstract
Maternally and paternally derived alleles can utilize different promoters, but allele-specific differences in cotranscriptional processes have not been reported. We show that alternative polyadenylation sites at a novel murine imprinted gene (H13) are utilized in an allele-specific manner. A differentially methylated CpG island separates polyA sites utilized on maternal and paternal alleles, and contains an internal promoter. Two genetic systems show that alleles lacking methylation generate truncated H13 transcripts that undergo internal polyadenylation. On methylated alleles, the internal promoter is inactive and elongation proceeds to downstream polyadenylation sites. This demonstrates that epigenetic modifications can influence utilization of alternative polyadenylation sites.
Keyword Polyadenylation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 71 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 74 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 08 Aug 2011, 09:26:01 EST by Susan Allen on behalf of Institute for Molecular Bioscience