Arachidonic-acid metabolism in human trabecular meshwork cells

Weinreb, R.N., Polansky, J.R., Alvarado, J.A. and Mitchell, M.D. (1988) Arachidonic-acid metabolism in human trabecular meshwork cells. Investigative Ophthalmology and Visual Science, 29 11: 1708-1712.

Author Weinreb, R.N.
Polansky, J.R.
Alvarado, J.A.
Mitchell, M.D.
Title Arachidonic-acid metabolism in human trabecular meshwork cells
Journal name Investigative Ophthalmology and Visual Science   Check publisher's open access policy
ISSN 0146-0404
Publication date 1988-11
Sub-type Article (original research)
Volume 29
Issue 11
Start page 1708
End page 1712
Total pages 5
Place of publication Rockville, MD, United States
Publisher Association for Research in Vision and Ophthalmology
Language eng
Formatted abstract
Prostaglandins and other eicosanoids in the trabecular meshwork may play important physiological and pharmacological roles in the aqueous outflow pathway. In the present studies, we employed [14C]-arachidonic acid to explore potentially important pathways for the production of eicosanoids in cultured human trabecular meshwork cells (HTM). In these cells, we demonstrated that prostaglandin E2 (PGE2) and PGF(2α) are major cyclooxygenase products, with some 6-keto-PGF(1α) also detected. The amount of radiolabelled PGE2 formed was substantially higher than the PGF(2α) formed in the early time periods. The amount of PGF(2α) in the culture media increased at a time when the amount of PGE2 was declining, suggesting a possible metabolic conversion between the prostaglandins. HTM produced a range of products of the lipoxygenase pathway. Products co-eluting with 5, 12, and 15-hydroxyeicosatetraenoic acids (HETEs) were detected, with 12 and 15-HETEs predominating. A large amount of radiolabelled products was detected also in peaks co-eluting with leukotriene B4 (LTB4) and an LTB4 degradation product. Biosynthesis of lipoxygenase products was markedly inhibited by BW 755c and partially inhibited by dexamethasone. These data emphasize that HTM cells are capable of converting arachidonic acid into a wider variety of biologically active products than previously recognized.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
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