Aging-related reduced release of LH-releasing hormone from hypothalamic granules

Rice, Gregory E., Cho, Grace and Barnea, Ayalla (1983) Aging-related reduced release of LH-releasing hormone from hypothalamic granules. Neurobiology of Aging, 4 3: 217-222. doi:10.1016/0197-4580(83)90023-4

Author Rice, Gregory E.
Cho, Grace
Barnea, Ayalla
Title Aging-related reduced release of LH-releasing hormone from hypothalamic granules
Journal name Neurobiology of Aging   Check publisher's open access policy
ISSN 0197-4580
Publication date 1983
Sub-type Article (original research)
DOI 10.1016/0197-4580(83)90023-4
Volume 4
Issue 3
Start page 217
End page 222
Total pages 6
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Language eng
Formatted abstract
The effects of aging on the secretory function of hypothalamicgranules containing luteinizing hormonereleasinghormone (LHRH) were examined, using chelated copper as a test substance. Granules prepared from old and young female rats were each incubated with various concentrations of CuATP and the kinetic constants of LHRH release estimated. The LHRH content of the hypothalamus of old female rats (3.4± 0.1 ng; mean±S.E.; n=3 determinations) was lower than that of young female rats (4.7±0.1 ng;n=3). CuATP-stimulated LHRH release (pg/hypothalamus) from granules isolated from old animals was significantly (p<0.001) lower than that from granules isolated from young animals; Vmax being 58.5±1.6 pg (n=36) and 76.7±2.7 pg (n=34) of LHRH released/min, respectively. In contrast, the fractional amount of CuATP-stimulated of LHRH from granules isolated from old and young rats was similar; Vmax being 60±0.5% and 62±1% of granule LHRH released respectively. In addition, the apparent Km for CuATP-stimulated release of LHRH from granules of old and youn rats was also similar, 5.1 and 5.5. μM copper, respectively. These results are suggestive that aging does not alter the responsiveness of LHRH-containing granules to a secretion-stimulus such as copper but it does alter the capacity (amount) of peptide released.
Keyword LHRH release
Secretory granules
Copper-LHRH release
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
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