Ovine allantoic fluid inhibition of prostaglandin synthesis in cotyledonary microsomes

Rice, G. E., Wong, M. H., Ralph, M. M. and Thorburn, G. D. (1987) Ovine allantoic fluid inhibition of prostaglandin synthesis in cotyledonary microsomes. Journal of Endocrinology, 114 2: 295-300. doi:10.1677/joe.0.1140295

Author Rice, G. E.
Wong, M. H.
Ralph, M. M.
Thorburn, G. D.
Title Ovine allantoic fluid inhibition of prostaglandin synthesis in cotyledonary microsomes
Journal name Journal of Endocrinology   Check publisher's open access policy
ISSN 0022-0795
Publication date 1987-08
Sub-type Article (original research)
DOI 10.1677/joe.0.1140295
Volume 114
Issue 2
Start page 295
End page 300
Total pages 6
Place of publication Bristol, United Kingdom
Publisher BioScientifica
Language eng
Formatted abstract
Inhibition of microsomal prostaglandin (PG) biosynthesis by allantoic fluid, obtained from ewes at 80-120 days of gestation, was examined. Inhibition of cotyledonary microsomal PGE2 and PGF(2α) biosynthesis by lyophilized allantoic fluid occurred in a dose-dependent manner. The concentration of allantoic fluid required to inhibit PGE2 and PGF(2α) production by 50% averaged 17.9 ± 3.2 (S.E.M.) mg dry weight/ml (n = 5). Microsomal PG biosynthesis was markedly enhanced by the addition of arachidonic acid (30 μmol/l). Synthesis of PGE2 and PGF(2α) was increased to 245 ± 65% and 184 ± 14% of control (P < 0.05, n = 5) respectively. Treatment of cotyledonary microsomes with porcine phospholipase A2 (PLA2; 0.125 units/ml) also stimulated PG synthesis, PGE2 increasing to 216 ± 27% and PGF(2α) to 172 ± 14% of control (P < 0.05, n = 5) respectively. Allantoic fluid (20 mg dry weight/ml) inhibited arachidonic acid-stimulated PG synthesis (PGE2 by 48.6 ± 13.8% and PGF(2α) by 44.2 ± 7.7%) and PLA2-stimulated PG synthesis (PGE2 by 60.6 ± 11.6% and PGF(2α) by 74.8 ± 8.5%). Allantoic fluid, however, did not affect PLA2-stimulated release of arachidonic acid from microsomes, thus negating the possibility that allantoic fluid suppresses PG synthesis by inhibiting PLA2 activity. These data indicate that allantoic fluid inhibits PG production at the level of PG synthase enzymes. Prostaglandin inhibitor(s) in allantoic fluid may play a role in maintaining uterine quiescence throughout gestation and its withdrawal, at term, may be involved in the initiation of labour.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
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