Ovine myometrial cytosol inhibits phospholipase A activity, in vitro

Rice, G. E., Wong, M. H. and Thorburn, G. D. (1987) Ovine myometrial cytosol inhibits phospholipase A activity, in vitro. Prostaglandins, 34 4: 563-578. doi:10.1016/0090-6980(87)90099-2


Author Rice, G. E.
Wong, M. H.
Thorburn, G. D.
Title Ovine myometrial cytosol inhibits phospholipase A activity, in vitro
Journal name Prostaglandins   Check publisher's open access policy
ISSN 0090-6980
Publication date 1987-10
Sub-type Article (original research)
DOI 10.1016/0090-6980(87)90099-2
Volume 34
Issue 4
Start page 563
End page 578
Total pages 16
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Language eng
Formatted abstract
Myometrium obtained from pregnant ewes (30-80 days gestation) contains a factor which inhibits phospholipase A2 (PLA2) activity. The activity of this moiety was assessed using an in vitro porcine pancreatic PLA2 assay system. Inhibitory activity was associated with a 35-45000 dalton molecular weight fraction, heat-labile, sensitive to protease degradation and did not partition into organic solvents. These data are indicative that PLA2-inhibitory activity resides in a protein moiety. Dixon-plot analysis of myometrial-inhibitory activity was indicative that the inhibition of PLA2 activity was of a non-competitive nature (Ki = 4.1 ± 0.7μg/ml, ca 118 nmol/1). Myometrial phospholipase-inhibitory protein(s) may be involved in the suppression of eicosanoid biosynthesis by the uterine tissues throughout gestation thus inhibiting uterine contractile activity.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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