Prostaglandin G/H synthase activity in ovine cotyledons: a gestational profile

Rice, G. E., Wong, M. H., Hollingworth, S. A. and Thorburn, G. D. (1990) Prostaglandin G/H synthase activity in ovine cotyledons: a gestational profile. Eicosanoids, 3 4: 231-236.

Author Rice, G. E.
Wong, M. H.
Hollingworth, S. A.
Thorburn, G. D.
Title Prostaglandin G/H synthase activity in ovine cotyledons: a gestational profile
Journal name Eicosanoids   Check publisher's open access policy
ISSN 0934-9820
Publication date 1990
Sub-type Article (original research)
Volume 3
Issue 4
Start page 231
End page 236
Total pages 6
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Formatted abstract
A polarographic oxygen electrode technique was used to quantify the activity of prostaglandin G/H synthase present in microsomes prepared from cotyledons of ewes at 74 to 147 days of gestation (n = 25). Microsomal oxygen consumption was stimulated by arachidonic acid and suppressed significantly (p less than 0.0001) by indomethacin, a specific inhibitor of prostaglandin G/H synthase. A residual indomethacin-incentive oxygen consumption was identified but the magnitude of this component did not change during gestation. During gestation prostaglandin G/H synthase (PGHS) activity, present in ovine cotyledons, increased 22-fold, from 0.6 +/- 0.19 at 70-80 days (n = 3) to 13.22 +/- 2.08 nmol O2/min/mg protein at greater than 145 days of gestation (n = 8; p less than 0.001). This gestational increase in the PGHS activity present in ovine cotyledons was not observed when fetal hypophysectomy was performed at 70-80 days of gestation. The data obtained in this study are consistent with the hypothesis that cotyledonary PGHS activity is increased during the second half of gestation and that this increase may be dependent upon fetal pituitary factors.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
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