Effect of high oxygen on placental function in short-term explant cultures

Reti, Nicole G., Lappas, Martha, Huppertz, Berthold, Riley, Clyde, Wlodek, Mary E., Henschke, Phil, Permezel, Michael and Rice, Gregory E. (2007) Effect of high oxygen on placental function in short-term explant cultures. Cell and Tissue Research, 328 3: 607-616. doi:10.1007/s00441-006-0375-1


Author Reti, Nicole G.
Lappas, Martha
Huppertz, Berthold
Riley, Clyde
Wlodek, Mary E.
Henschke, Phil
Permezel, Michael
Rice, Gregory E.
Title Effect of high oxygen on placental function in short-term explant cultures
Journal name Cell and Tissue Research   Check publisher's open access policy
ISSN 0302-766X
1432-0878
Publication date 2007-06
Sub-type Article (original research)
DOI 10.1007/s00441-006-0375-1
Volume 328
Issue 3
Start page 607
End page 616
Total pages 10
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Formatted abstract
Ex situ culture of human gestational tissues has been routinely used as a model to investigate tissue function. The objective of this study was to determine the effect of varying oxygen concentrations on human term placental explants over a 24-h time period. Specifically, the effect of incubating placental explants in oxygen concentrations of 8%, 21% or 95% on tissue viability, metabolism and cell death was measured by assessing glucose consumption, lactate production, release of lactate dehydrogenase, parathyroid hormone-related protein (PTHrP), tumour necrosis factor-alpha (TNF-α) and 8-isoprostane, immunoreactivity for cleaved-caspase-9 and immunohistochemistry for the caspase-3-cleaved cytokeratin-18 neoepitope, M30. Exposure to higher oxygen concentrations significantly increased the rates of glucose consumption and lactate production. Apoptosis was significantly increased under conditions of higher oxygen as evidenced by increased M30 in placental explant sections. Similarly, hyperoxia significantly increased the releases of PTHrP, TNF-α and 8-isoprostane. Thus, incubation of placental explants with oxygen concentrations of 95% and, to a lesser extent, 21% oxygen was associated with the modulation of multiple cellular response pathways including those associated with tissue viability and cell death. These data are consistent with the hypothesis that hyperoxia activates pathways and mechanisms involved in cellular metabolism, necrosis and apoptosis, thereby shifting the balance from a steady state towards cell death.
Keyword term placental explants
short-term cultures
oxidant stress
metabolite activity
apoptosis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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