Regulation of oxytocin secretion by the ovine corpus luteum: Effect of activators of protein kinase C

Hirst, J. J., Rice, G. E., Jenkin, G. and Thorburn, G. D. (1990) Regulation of oxytocin secretion by the ovine corpus luteum: Effect of activators of protein kinase C. Journal of Endocrinology, 124 2: 225-232. doi:10.1677/joe.0.1240225


Author Hirst, J. J.
Rice, G. E.
Jenkin, G.
Thorburn, G. D.
Title Regulation of oxytocin secretion by the ovine corpus luteum: Effect of activators of protein kinase C
Journal name Journal of Endocrinology   Check publisher's open access policy
ISSN 0022-0795
1479-6805
Publication date 1990-02
Sub-type Article (original research)
DOI 10.1677/joe.0.1240225
Volume 124
Issue 2
Start page 225
End page 232
Total pages 8
Place of publication Bristol, United Kingdom
Publisher BioScientifica
Language eng
Formatted abstract
The effect of protein kinase C activation and dibutyryl cyclic AMP on oxytocin secretion by ovine luteal tissue slices was investigated. Several putative regulators of luteal oxytocin secretion were also examined. Oxytocin was secreted by luteal tissue slices at a basal rate of 234.4 ± 32.8 pmol/g per h (n = 24) during 60-min incubations. Activators of protein kinase C: phorbol 12,13-dibutyrate (n = 8), phorbol 12-myristate,13-acetate (n = 4) and 1,2-didecanoylglycerol (n = 5), caused a dose-dependent stimulation of oxytocin secretion in the presence of a calcium ionophore (A23187; 0.2 μmol/l). Phospholipase C (PLC; 50-250 units/l) also caused a dose-dependent stimulation of oxytocin secretion by luteal slices. Phospholipase C-stimulated oxytocin secretion was potentiated by the addition of an inhibitor of diacylglycerol kinase (R59022; n = 4). These data suggest that the activation of protein kinase C has a role in the stimulation of luteal oxytocin secretion. The results are also consistent with the involvement of protein kinase C in PLC-stimulated oxytocin secretion. The cyclic AMP second messenger system does not appear to be involved in the control of oxytocin secretion by the corpus luteum.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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