Interleukin-8 release from human gestational tissue explants: The effects of lipopolysaccharide and cytokines

Laham, N., Brennecke, S. P. and Rice, G. E. (1997) Interleukin-8 release from human gestational tissue explants: The effects of lipopolysaccharide and cytokines. Biology of Reproduction, 57 3: 616-620. doi:10.1095/biolreprod57.3.616


Author Laham, N.
Brennecke, S. P.
Rice, G. E.
Title Interleukin-8 release from human gestational tissue explants: The effects of lipopolysaccharide and cytokines
Journal name Biology of Reproduction   Check publisher's open access policy
ISSN 0006-3363
1529-7268
Publication date 1997-09
Sub-type Article (original research)
DOI 10.1095/biolreprod57.3.616
Volume 57
Issue 3
Start page 616
End page 620
Total pages 5
Place of publication Madison, United States
Publisher Society for the Study of Reproduction
Language eng
Formatted abstract
Interleukin (IL)-8 is a chemotactic cytokine that has been implicated in the etiology of infection-induced and normal human labor. In particular, IL- 8 has been implicated in the processes of cervical ripening and rupture of fetal membranes because of its role in neutrophil activation and release of cellular matrix remodeling enzymes. In this study, we tested the hypotheses that IL-8 is released locally in the intrauterine environment from human amnion, choriodecidua, and placenta, and that IL-8 release from these tissues is increased by bacterial endotoxin, lipopolysaccharides (LPS), and inflammatory cytokines. IL-8 was released from human amnion, choriodecidual, and placental explants, with choriodecidua demonstrating the most abundant release. IL-8 release was significantly (multiple analysis of variance, p < 0.05) increased by LPS in a time- and dose-dependent manner from both choriodecidual and placental explants, but not from amnion explants. In addition, IL-1α (0.28 nM) and tumor necrosis factor α (TNFα, 10 nM) significantly (Student's t-test, p < 0.05) increased IL-8 release from placental explants 2-to 3-fold. These studies establish that the amnion, choriodecidua, and placenta are a source of IL-8 and demonstrate tissue- specific and differential regulation of IL-8 release by LPS, IL-1α, and TNF- α. These data support a role for IL-8 in a cascade of inflammatory events initiated by an intrauterine infection and resulting in activation of the labor process.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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