Expression and localization of alpha(v)beta(6) integrin in extraplacental fetal membranes: Possible role in human parturition

Ahmed, Nuzhat, Riley, Clyde, Oliva, Karen, Barker, Gillian, Quinn, Mark A. and Rice, Greg E. (2004) Expression and localization of alpha(v)beta(6) integrin in extraplacental fetal membranes: Possible role in human parturition. Molecular Human Reproduction, 10 3: 173-179. doi:10.1093/molehr/gah025


Author Ahmed, Nuzhat
Riley, Clyde
Oliva, Karen
Barker, Gillian
Quinn, Mark A.
Rice, Greg E.
Title Expression and localization of alpha(v)beta(6) integrin in extraplacental fetal membranes: Possible role in human parturition
Journal name Molecular Human Reproduction   Check publisher's open access policy
ISSN 1360-9947
1460-2407
Publication date 2004-03-01
Sub-type Article (original research)
DOI 10.1093/molehr/gah025
Volume 10
Issue 3
Start page 173
End page 179
Total pages 7
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Language eng
Formatted abstract
Successful outcome of human parturition is dependent upon extensive remodelling of the extracellular matrix (ECM) of the cervix, uterus and fetal membranes, a process that involves adhesion molecules and is also common in tumour invasion and metastasis. To elucidate the role of integrins in human parturition, this study characterizes the expression of the tumour‐associated αvβ6 integrin in human placenta and extraplacental membranes. Immunohistochemical analysis of the placenta and fetal membranes from normal vaginal deliveries (NVD) (n = 10) exhibited strong intensity of staining for αvβ6 integrin (3 = dark brown) in the epithelial layer of the amnion. Weak immunohistochemical staining of αvβ6 integrin (1 = pale brown) was detected in the chorion and at the decidual edge. These results were consistent with the immunodetection of αvβ6 integrin by western blot analysis that showed 4‐fold enhanced expression in the amnion compared to chorion of both NVD and term elective caesarean section (CS) deliveries. Even though there was no difference in the extent of immunohistochemical staining of αvβ6 integrin between the amnion of NVD and CS groups, significantly higher intensity of staining was observed in the NVD amniotic epithelium compared to that of CS (n = 10) (χ2 = 10.25, P = 0.0059). Western blot analysis of the fetal membranes showed no differences in the expression of αvβ6 integrin between the NVD and CS groups. Gelatin zymography demonstrated the presence of pro‐matrix metalloprotein‐9 (MMP‐9) and pro‐MMP‐2 in the amnion and chorion of NVD, whereas in CS only the presence of pro‐MMP‐2 was observed. These results suggest that in term pregnancy, human fetal membranes express αvβ6 integrin and that the expression is significantly higher in amnion compared to chorion. The fact that enhanced expression of αvβ6 integrin in fetal membranes correlates with the expression of pro‐MMP‐9 in NVD is consistent with the invasive role of the integrin in cancer and suggests that the molecule may have a proteolytic role in the initiation and progression of labour.
Keyword Amnion
Chorion
Integrin
Parturition
Placenta
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 8 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 15 Jul 2011, 23:48:07 EST by System User on behalf of UQ Centre for Clinical Research