Leptin and adiponectin stimulate the release of proinflammatory cytokines and prostaglandins from human placenta and maternal adipose tissue via nuclear factor-kappa B, peroxisomal proliferator-activated receptor-gamma and extracellularly regulated kinase

Lappas, Martha, Permezel, Michael and Rice, Gregory E. (2005) Leptin and adiponectin stimulate the release of proinflammatory cytokines and prostaglandins from human placenta and maternal adipose tissue via nuclear factor-kappa B, peroxisomal proliferator-activated receptor-gamma and extracellularly regulated kinase 1/2. Endocrinology, 146 8: 3334-3342. doi:10.1210/en.2005-0406


Author Lappas, Martha
Permezel, Michael
Rice, Gregory E.
Title Leptin and adiponectin stimulate the release of proinflammatory cytokines and prostaglandins from human placenta and maternal adipose tissue via nuclear factor-kappa B, peroxisomal proliferator-activated receptor-gamma and extracellularly regulated kinase 1/2
Formatted title
Leptin and adiponectin stimulate the release of proinflammatory cytokines and prostaglandins from human placenta and maternal adipose tissue via nuclear factor-κB, peroxisomal proliferator-activated receptor-γ and extracellularly regulated kinase 1/2
Journal name Endocrinology   Check publisher's open access policy
ISSN 0013-7227
1945-7170
Publication date 2005-08
Sub-type Article (original research)
DOI 10.1210/en.2005-0406
Volume 146
Issue 8
Start page 3334
End page 3342
Total pages 9
Place of publication Chevy Chase, MD, United States
Publisher The Endocrine Society
Language eng
Formatted abstract
Beyond their effects on central metabolic functions, leptin, resistin, and adiponectin have profound effects on a number of other physiologic processes, including immune function and inflammation. Although leptin, resistin, and adiponectin are produced in human placenta and adipose tissue, their immunoregulatory actions in these tissues are not known. Therefore, the aim of this study was to determine the effect of leptin, resistin, and adiponectin on the release of proinflammatory mediators in human placenta and sc adipose tissue. Samples were obtained from normal pregnancies at the time of cesarean section. Tissue explants (n = 5) were incubated in the absence (basal control) or presence of a leptin (1, 10, and 100 ng/ml), resistin (1, 10, and 100 ng/ml), and adiponectin (0.1 and 0.5 μg/ml). After 6 h incubation, the medium was collected, and the release of IL-1β, IL-6, TNFα, prostaglandin (PG)F and PGE2 was quantified by ELISA. There was no effect of resistin on proinflammatory cytokine or prostaglandin release; however, leptin at 100 ng/ml and adiponectin at 0.1 and/or 0.5 μg/ml significantly increased the release of IL-1β, IL-6, TNFα, and PGE2 from human placenta and adipose tissue. Although both leptin and adiponectin significantly increased PGF release from human placenta, there was no effect of these hormones on PGF release from adipose tissue. Furthermore, this leptin- and adiponectin-induced proinflammatory response could be abrogated by treatment with the antiinflammatory ERK1/2 MAPK inhibitor U0126, the peroxisomal proliferator-activated receptor-γ ligand troglitazone, and the nuclear factor-κB inhibitor BAY 11-7082. Collectively, these data indicate that leptin and adiponectin activate proinflammatory cytokine release and phospholipid metabolism in human placenta and adipose tissue, and antiinflammatory agents can abrogate leptin- and adiponectin-induced inflammation.
Keyword Complement-Related Protein
Human Gestational Tissues
Bone-Marrow Cultures
In-Vitro
Skeletal-Muscle
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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