Protein profiling the effects of in vitro hyperoxic exposure on fetal rabbit lung

Henschke, Phillip, Vorum, Henrik, Honore, Bent and Rice, Gregory Edward (2006) Protein profiling the effects of in vitro hyperoxic exposure on fetal rabbit lung. Proteomics, 6 6: 1957-1962. doi:10.1002/pmic.200500245


Author Henschke, Phillip
Vorum, Henrik
Honore, Bent
Rice, Gregory Edward
Title Protein profiling the effects of in vitro hyperoxic exposure on fetal rabbit lung
Journal name Proteomics   Check publisher's open access policy
ISSN 1615-9853
1615-9861
Publication date 2006-03
Sub-type Article (original research)
DOI 10.1002/pmic.200500245
Volume 6
Issue 6
Start page 1957
End page 1962
Total pages 6
Place of publication Weinheim, Germany
Publisher Wiley
Language eng
Formatted abstract
The aim of this study was to test the hypothesis that acute in vitro exposure of prematurely delivered fetal rabbit lungs to hyperoxic conditions will induce the expression of an adaptive cassette of proteins that mediates antioxidant and inflammatory processes. To test this hypothesis, ex situ fetal rabbit lung explants were prepared from New Zealand white rabbits delivered by cesarean section on day 29 of gestation and incubated under air (21% O2; 5% CO2) or hyperoxic (95% O2; 5% CO2) atmospheres. Total tissue protein was extracted following incubation and subjected to 2-DE. Using this technique, 1500–2000 protein spots were resolved per gel. Treatment-dependent, differentially expressed proteins were identified by image analysis (Melanie II) and MALDI-TOF MS and MALDI-MS/MS. The analysis identified 12 protein spots that were differentially expressed by 1.5-fold or more (p<0.05) by exposure to hyperoxic conditions. Six of these differentially expressed proteins were identified as vimentin, annexin I, inorganic pyrophosphatase, prohibitin, an N-terminal fragment of ATP synthase and heat shock protein 27. The data obtained are consistent with the roles of these proteins in mediating cellular response to oxidative stress and in regulating cell proliferation.
Keyword 2-De
Fetal rabbit lung
Hyperoxia
Oxidative Stress
Prematurity
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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