Pre-labour Fetal Membranes Overlying the Cervix Display Alterations in Inflammation and NF-kappa B Signalling Pathways

Lappas, M., Odumetse, T. L., Riley, C., Reti, N. G., Holdsworth-Carson, S. J., Rice, G. E. and Permezel, M. (2008) Pre-labour Fetal Membranes Overlying the Cervix Display Alterations in Inflammation and NF-kappa B Signalling Pathways. Placenta, 29 12: 995-1002. doi:10.1016/j.placenta.2008.09.010


Author Lappas, M.
Odumetse, T. L.
Riley, C.
Reti, N. G.
Holdsworth-Carson, S. J.
Rice, G. E.
Permezel, M.
Title Pre-labour Fetal Membranes Overlying the Cervix Display Alterations in Inflammation and NF-kappa B Signalling Pathways
Formatted title
Pre-labour Fetal Membranes Overlying the Cervix Display Alterations in Inflammation and NF-κB Signalling Pathways
Journal name Placenta   Check publisher's open access policy
ISSN 0143-4004
1532-3102
Publication date 2008-12
Sub-type Article (original research)
DOI 10.1016/j.placenta.2008.09.010
Volume 29
Issue 12
Start page 995
End page 1002
Total pages 8
Place of publication Oxford, United Kindgom
Publisher Elsevier
Language eng
Formatted abstract
To generate new insights into the process of fetal membrane rupture, we have developed a technique whereby fetal membranes overlying the cervix (i.e. supracervical site, SCS) are tagged in women undergoing term elective Caesarean section. The specific aim is to determine the effect of supracervical apposition on the release of inflammatory mediators and NF-κB signalling in pre-labour fetal membranes. Fetal membranes were collected from both the SCS and from a distal site (DS). The level of NF-κB proteins and its transcriptional co-activator protein CBP and p300 was determined by Western blotting and/or immunohistochemistry (IHC), and cytokine and prostaglandin release was quantified by enzyme immunoassay. Tissues obtained before labour at term possess an area of the fetal membranes (i.e. supracervical site) that exhibit decreased release of IL-1β, IL-6, IL-8, TNF-α and PGE2. IHC revealed that NF-κB signalling proteins, CBP and p300 were significantly increased in SC fetal membranes compared to distal membranes. In summary, data from this study confirm that supracervical fetal membranes display altered structural and biochemical characteristics.
Keyword Rupture of fetal membranes
Prom
Cytokines
NF-kappa B
Human Decidual Macrophages
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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