Peroxisome Proliferator-activated Receptors and Retinoid X Receptor-alpha in Term Human Gestational Tissues: Tissue Specific and Labour-associated Changes

Holdsworth-Carson, S. J., Permezel, M., Riley, C., Rice, G. E. and Lappas, M. (2009) Peroxisome Proliferator-activated Receptors and Retinoid X Receptor-alpha in Term Human Gestational Tissues: Tissue Specific and Labour-associated Changes. Placenta, 30 2: 176-186. doi:10.1016/j.placenta.2008.11.013


Author Holdsworth-Carson, S. J.
Permezel, M.
Riley, C.
Rice, G. E.
Lappas, M.
Title Peroxisome Proliferator-activated Receptors and Retinoid X Receptor-alpha in Term Human Gestational Tissues: Tissue Specific and Labour-associated Changes
Journal name Placenta   Check publisher's open access policy
ISSN 0143-4004
1532-3102
Publication date 2009-02
Sub-type Article (original research)
DOI 10.1016/j.placenta.2008.11.013
Volume 30
Issue 2
Start page 176
End page 186
Total pages 11
Place of publication Oxford, United Kingdom
Publisher Elsevier
Language eng
Formatted abstract
Peroxisome proliferator-activated receptors (PPARs) and their transcriptional partner retinoid X receptor (RXR) are involved in transcriptionally regulating the events that contribute to the control of parturition in humans. Definitive data, however, are lacking with respect to PPAR and RXR expression and activation during term labour in human gestational tissues. The aim of this study, therefore, was to identify tissue and labour-associated changes of PPAR isoforms (α, δ and γ) and RXRα in placenta, amnion and choriodecidua. Gestational tissues from term non-labouring women were used for immunohistochemistry localisation and confirmation studies of PPAR isoforms (α, δ and γ) and RXRα. Human gestational tissues were then collected from term women not-in-labour (NIL) (elective Caesarean section), in-labour (IL) (emergency Caesarean section) and post-labour (PL) (normal vaginal delivery). Quantitative RT-PCR (qRT-PCR) and Western blotting were employed to study mRNA and protein expression profiles respectively. Significantly higher mRNA expression was observed in placental tissues taken from women in labour (PPARδ, PPARγ and RXRα). Elevated PPARδ and RXRα mRNA expression in fetal membranes was also associated with being in labour. In contrast, PPARγ mRNA in the amnion was decreased with term PL compared to NIL. In placenta, PPARα, PPARδ and PPARγ protein expression was significantly increased in the IL group compared to the NIL or PL group. There was no significant difference in PPAR or RXRα protein expression in both amnion and choriodecidua between the three labour groups. PPAR (α and γ) transcription factor DNA binding activity was found to decline IL compared to NIL and PL in the placenta. PPARδ DNA binding activity also decreased in the choriodecidua IL compared to PL. In amnion, PPARα DNA binding activity was found to be higher IL compared to NIL. In conclusion, term human labour is associated with changes in expression and activity of PPAR isoforms and its transcription partner, RXRα. This data is consistent with the hypothesis that PPAR:RXR are involved in regulating of the processes of human term parturition.
Keyword Term labour
Peroxisome proliferator-activated receptors
Retinoid X receptor
Placenta and fetal membranes
Factor-Kappa-B
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 15 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 14 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 15 Jul 2011, 13:23:13 EST by System User on behalf of UQ Centre for Clinical Research