Preterm and infection-driven preterm labor: the role of peroxisome proliferator-activated receptors and retinoid X receptor

Holdsworth-Carson, Sarah J., Permezel, Michael, Rice, Greg E. and Lappas, Martha (2009) Preterm and infection-driven preterm labor: the role of peroxisome proliferator-activated receptors and retinoid X receptor. Reproduction, 137 6: 1007-1015. doi:10.1530/REP-08-0496


Author Holdsworth-Carson, Sarah J.
Permezel, Michael
Rice, Greg E.
Lappas, Martha
Title Preterm and infection-driven preterm labor: the role of peroxisome proliferator-activated receptors and retinoid X receptor
Journal name Reproduction   Check publisher's open access policy
ISSN 1470-1626
1741-7899
Publication date 2009-06
Sub-type Article (original research)
DOI 10.1530/REP-08-0496
Volume 137
Issue 6
Start page 1007
End page 1015
Total pages 9
Place of publication Bristol, United Kingdom
Publisher BioScientifica
Language eng
Formatted abstract
Approximately 8% of births are complicated by preterm delivery. To improve neonatal outcomes, a greater understanding of the mechanisms surrounding preterm parturition is required. Peroxisome proliferator-activated receptors (PPARs) have been implicated in the regulation of labor at term where they exhibit anti-inflammatory properties. Thus, we hypothesize that dysregulation of PPAR expression and activity may be associated with preterm labor and infection-associated preterm labor. The aim of this study was to compare the expression and activity of PPARs and the expression of retinoid X-receptor α (RXRA) in gestational tissues from term and preterm deliveries, and from infection-associated preterm deliveries. Quantitative RT-PCR, western blotting and activity ELISA were used to study expression and DNA binding profiles. Compared with term, preterm parturition was associated with an increased expression of PPAR δ (PPARD; mRNA and protein), PPAR γ (PPARG; protein) and RXRA (protein) in the placenta and PPARD (mRNA and protein) and RXRA (mRNA) in the choriodecidua. There was, however, no change in preterm PPAR DNA binding activity compared with term. Preterm chorioamnionitis (CAM) demonstrated protein degradation in the choriodecidua and was associated with a decline in the mRNA expression of PPAR α (PPARA) and RXRA compared with uninfected preterm cases. PPAR DNA binding activity increased in the placenta (PPARD and PPARG) and decreased in the amnion (PPARA and PPARG) in association with preterm CAM. In conclusion, idiopathic preterm deliveries were associated with an increase in PPAR:RXR expression and preterm CAM was associated with a decrease in PPAR:RXR expression and tissue-specific alterations in transcriptional activity. The reasons for such dysregulation remain to be determined; however, the data are consistent with the hypothesis that PPARs may play a role in preterm labor and infection-complicated preterm deliveries.
Keyword Human Gestational Tissues
Factor-Kappa-B
Nuclear Hormone-Receptors
Human Fetal Membranes
Ppar-Gamma
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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