The identification of differentially expressed proteins associated with human labour at term: 1D and 2D differential in gel electrophoresis (DIGE) coupled with mass spectrometry

Lappas, Martha, Oliva, Karen, Bailey, Mark J., Permezel, Michael and Rice, Greg E. (2011). The identification of differentially expressed proteins associated with human labour at term: 1D and 2D differential in gel electrophoresis (DIGE) coupled with mass spectrometry. In: Abstracts of the 58th Annual Scientific Meeting of the Society for Gynecologic Investigation. 58th Annual Scientific Meeting of the Society for Gynecologic Investigation, Miami Beach FL, United States, (346A-346A). 16-19 March 2011. doi:10.1177/193371912011183s067


Author Lappas, Martha
Oliva, Karen
Bailey, Mark J.
Permezel, Michael
Rice, Greg E.
Title of paper The identification of differentially expressed proteins associated with human labour at term: 1D and 2D differential in gel electrophoresis (DIGE) coupled with mass spectrometry
Conference name 58th Annual Scientific Meeting of the Society for Gynecologic Investigation
Conference location Miami Beach FL, United States
Conference dates 16-19 March 2011
Proceedings title Abstracts of the 58th Annual Scientific Meeting of the Society for Gynecologic Investigation   Check publisher's open access policy
Journal name Reproductive Sciences   Check publisher's open access policy
Place of Publication Sage Publications
Publisher Thousand Oaks CA, United States
Publication Year 2011
Sub-type Poster
DOI 10.1177/193371912011183s067
Open Access Status
ISSN 1933-7191
1933-7205
Volume 18
Issue 3
Start page 346A
End page 346A
Total pages 1
Collection year 2012
Language eng
Formatted Abstract/Summary
BACKGROUND: The aim of this study was to determine the effect of human labour on the placental proteome.

METHODS: Human placentae were obtained from women who delivered healthy, singleton infants at term at elective Caesarean section (no labour) and after spontaneous labour and normal vaginal delivery (n=6 per group). We used liquid phase isoelectric focusing (OFFGEL ™) coupled with one-dimensional difference gel electrophoresis (1D-DIGE) and conventional 2D-DIGE, followed by mass spectrometry (MS) of selected protein spots. Western blotting and quantitative RT-PCR (qRT-PCR) was used for confirmation of spot differences.

RESULTS: The 2D-DIGE technology allowed the analysis of ∼637 protein spots in the comparative study of no labour versus labour. Fourteen protein spots were differentially expressed (P<0.05) of these, nine unique proteins were identified by MS. The proteins were alpha-2-macroglobulin, transitional endoplasmic reticulum ATPase, guanine nucleotide-binding protein subunit beta-2, mitochondrial inner membrane protein, alpha-1-antitrypsin, myosin light polypeptide 6, endoplasmin, myosin regulatory light polypeptide 9 and neutral alpha-glucosidase AB. OFFGEL 1D-DIGE confi rmed the differential expression of the above proteins.

CONCLUSIONS: Proteomic analysis of human placenta reveals differential expression of several proteins in association with term spontaneous labour. The application of this novel methodology adds further evidence to support the role of proteolysis, proliferation, apoptosis and infl ammation in the pathogenesis of human labour.
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Published under Poster Session: Basic Parturition/Prematurity : Scientific Abstracts of the SGI 58th Annual Scientific Meeting: Reproduction and Regenerative Medicine.

Document type: Conference Paper
Collection: UQ Centre for Clinical Research Publications
 
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