Epigenetic regulation of pro-inflammatory cytokines and prostaglandins in primary amnion cells

Lappas, Martha, Dellios, Nicole, Mitchell, Murray D., Permezel, Michael and Rice, Gregory E. (2011). Epigenetic regulation of pro-inflammatory cytokines and prostaglandins in primary amnion cells. In: Abstracts of the 58th Annual Scientific Meeting of the Society for Gynecologic Investigation. 58th Annual Scientific Meeting of the Society for Gynecologic Investigation, Miami Beach FL, United States, (298A-298A). 16-19 March 2011. doi:10.1177/193371912011183s067


Author Lappas, Martha
Dellios, Nicole
Mitchell, Murray D.
Permezel, Michael
Rice, Gregory E.
Title of paper Epigenetic regulation of pro-inflammatory cytokines and prostaglandins in primary amnion cells
Conference name 58th Annual Scientific Meeting of the Society for Gynecologic Investigation
Conference location Miami Beach FL, United States
Conference dates 16-19 March 2011
Proceedings title Abstracts of the 58th Annual Scientific Meeting of the Society for Gynecologic Investigation   Check publisher's open access policy
Journal name Reproductive Sciences   Check publisher's open access policy
Place of Publication Thousand Oaks CA, United States
Publisher Sage Publications
Publication Year 2011
Sub-type Poster
DOI 10.1177/193371912011183s067
ISSN 1933-7191
1933-7205
Volume 18
Issue 3, Supp.
Start page 298A
End page 298A
Total pages 1
Collection year 2012
Language eng
Formatted Abstract/Summary
Background: Human term and preterm parturition is associated with increased production of pro-infl ammatory cytokines (e.g. IL-6) and prostaglandins from human gestational tissues. Pro-infl ammatory cytokines stimulate the ongoing release of prostaglandins. Prostaglandins, especially PGE2 and PGF2α, are recognised as proximal factors that promote myometrial contractility and cervical dilatation. Aberrant DNA methylation patterns and histone modifications have been associated with dysfunction and disease. There is, however, a paucity of data concerning the role of DNA methylation and histone modification in the process and consequences of human labour.

Objective: To determine the effects of the inhibitor of DNA methylation 5-aza-2-deoxycytidine (AZA) and the inhibitor of histone deacetylation trichostatin A (TSA) on IL-1β-stimulated pro-infl ammatory cytokine and prostaglandin release in primary amnion epithelial cells.

Methods:
Primary amnion epithelial cultures (n=5) were isolated from term amnion and treated with 5μM AZA or 300nM TSA in the absence or presence of 10ng/ml IL-1β for 24h. Media were analysed for IL-6, IL-8, PGE2 and PGF2α content by ELISA.

Results:
IL-1β treatment signifi cantly increased the release of IL-6, IL-8, PGE2 and PGF2α from primary amnion cells. There was, however, no effect of AZA on IL-1β induced cytokine or prostaglandin release. Although there was no effect of TSA on IL-1β induced IL-8 production, it signifi cantly attenuated IL-1β stimulated IL-6, PGE2 and PGF2α.

Conclusions:
Our data demonstrate that the release of prostaglandins in human primary amnion cells is inhibited by TSA treatment following IL-1β stimulation. In contrast, IL-1β induced pro-infl ammatory cytokine release is differentially regulated by the inhibitor of histone deacetylation TSA. Collectively, these preliminary data suggests a role for epigenetic regulation of pro-infl ammatory mediators through histone modifi cations. On the other hand, pro-infl ammatory cytokine and prostaglandin release is not regulated by this method of inhibition of DNA methylation.
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Published under Poster Session: Basic Parturition/Prematurity : Scientific Abstracts of the SGI 58th Annual Scientific Meeting: Reproduction and Regenerative Medicine.

Document type: Conference Paper
Collections: UQ Centre for Clinical Research Publications
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