Parallel origins of duplications and the formation of pseudogenes in mitochondrial DNA from parthenogenetic lizards (Heteronotia binoei; gekkonidae)

Zevering, C. E., Heideman, A. and Sturm, R. A. (1991) Parallel origins of duplications and the formation of pseudogenes in mitochondrial DNA from parthenogenetic lizards (Heteronotia binoei; gekkonidae). Journal of Molecular Evolution, 33 5: 431-441. doi:10.1007/BF02103135


Author Zevering, C. E.
Heideman, A.
Sturm, R. A.
Title Parallel origins of duplications and the formation of pseudogenes in mitochondrial DNA from parthenogenetic lizards (Heteronotia binoei; gekkonidae)
Journal name Journal of Molecular Evolution   Check publisher's open access policy
ISSN 0022-2844
1432-1432
Publication date 1991-11
Sub-type Article (original research)
DOI 10.1007/BF02103135
Volume 33
Issue 5
Start page 431
End page 441
Total pages 11
Place of publication Secaucus, NJ, United States
Publisher Springer
Language eng
Abstract Analysis of mitochondrial DNAs (mtDNAs) from parthenogenetic lizards of the Heteronotia binoei complex with restriction enzymes revealed an ~5-kb addition present in all 77 individuals. Cleavage site mapping suggested the presence of a direct tandem duplication spanning the 16S and 12S rRNA genes, the control region and most, if not all, of the gene for the subunit 1 of NADH dehydrogenase (ND1). The location of the duplication was confirmed by Southern hybridization. A restriction enzyme survey provided evidence for modifications to each copy of the duplicated sequence, including four large deletions. Each gene affected by a deletion was complemented by an intact version in the other copy of the sequence, although for one gene the functional copy was heteroplasmic for another deletion. Sequencing of a fragment from one copy of the duplication which encompassed the tRNA(leu(UUR)) and parts of the 16S rRNA and ND1 genes, revealed mutations expected to disrupt function. Thus, evolution subsequent to the duplication event has resulted in mitochondrial pseudogenes. The presence of duplications in all of these parthenogens, but not among representatives of their maternal sexual ancestors, suggests that the duplications arose in the parthenogenetic form. This provides the second instance in H. binoei of mtDNA duplication associated with the transition from sexual to parthenogenetic reproduction. The increased incidence of duplications in parthenogenetic lizards may be caused by errors in mtDNA replication due to either polyploidy or hybridity of their nuclear genomes.
Keyword Restriction mapping
Southern Blotting
Cloning and sequencing
Australian geckos
Control region
16S-ribosomal-rna
NADH dehydrogenase-1
Nucleocytoplasmic interactions
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 36 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 31 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 08 Jul 2011, 10:31:49 EST by System User on behalf of Institute for Molecular Bioscience