In vivo and in vitro expression of octamer binding proteins in human melanoma metastases, brain tissue, and fibroblasts

Thomson, J. Angus F., Parsons, Peter G. and Sturm, Richard A. (1993) In vivo and in vitro expression of octamer binding proteins in human melanoma metastases, brain tissue, and fibroblasts. Pigment Cell Research, 6 1: 13-22. doi:10.1111/j.1600-0749.1993.tb00576.x


Author Thomson, J. Angus F.
Parsons, Peter G.
Sturm, Richard A.
Title In vivo and in vitro expression of octamer binding proteins in human melanoma metastases, brain tissue, and fibroblasts
Journal name Pigment Cell Research   Check publisher's open access policy
ISSN 0893-5785
1600-0749
Publication date 1993-02
Sub-type Article (original research)
DOI 10.1111/j.1600-0749.1993.tb00576.x
Volume 6
Issue 1
Start page 13
End page 22
Total pages 10
Place of publication Malden, MA, United States
Publisher Wiley-Blackwell
Language eng
Abstract The pattern of octamer sequence-specific DNA binding proteins expressed in human melanoma was examined in nuclear extracts of seven surgically-isolated tumors, short-term cultures of these tumors, and 25 human melanoma cell lines to determine the in vivo and in vitro distribution of the melanocytic-associated Oct-M1 and Oct-M2 octamer binding activities. In the biopsy tissue and cultured melanoma cells of a metastasis from the cerebellum, two other binding activities (N-Oct-2 and N-Oct-6) in addition to the Oct-M1, Oct-M2 and the generally expressed Oct-1 protein were detected; this profile was consistent with that seen in normal human and mouse brain tissue. Melanoma tissue removed from lymph nodes and cell lines established from them also showed Oct-1, Oct-M1, Oct-M2, and N-Oct-2. N-Oct-2 was distinguished from the comigrating Oct-2A activity by failure to react with Oct-2A-specific antibody. All but one of the 25 melanoma cell lines exhibited Oct-1, Oct-M1, and Oct-M2 and/or N-Oct-2 activity, whereas cultured normal melanocytes expressed only Oct-1 and Oct-M1. In contrast to murine fibroblasts, which express only Oct-1, human fibroblast strains also expressed Oct-2A binding activity, which was confirmed by reactivity with Oct-2A antibody and the presence of Oct-2A mRNA and indicated that Oct-2A has a more general role than that of a lymphoid-specific transcription factor. Overall, the results indicate that expression of neural-specific Oct factors in human melanoma is (1) aberrant compared with normal melanocytes, (2) can be modulated by the surrounding tissue in a brain metastasis, and (3) may be part of the altered program of differentiation accompanying transformation.
Keyword Melanoma metastasis
Transcription factor
Oct-M
N-Oct
Oct-2
B-Cell
Pou-Domain
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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