The recycling endosome protein Rab17 regulates melanocytic filopodia formation and melanosome trafficking

Beaumont, Kimberley A., Hamilton, Nicholas A., Moores, Matthew T., Brown, Darren L., Ohbayashi, Norihiko, Cairncross, Oliver, Cook, Anthony L., Smith, Aaron G., Misaki, Ryo, Fukuda, Mitsunori, Taguchi, Tomohiko, Sturm, Richard A. and Stow, Jennifer L. (2011) The recycling endosome protein Rab17 regulates melanocytic filopodia formation and melanosome trafficking. Traffic, 12 5: 627-643. doi:10.1111/j.1600-0854.2011.01172.x


Author Beaumont, Kimberley A.
Hamilton, Nicholas A.
Moores, Matthew T.
Brown, Darren L.
Ohbayashi, Norihiko
Cairncross, Oliver
Cook, Anthony L.
Smith, Aaron G.
Misaki, Ryo
Fukuda, Mitsunori
Taguchi, Tomohiko
Sturm, Richard A.
Stow, Jennifer L.
Title The recycling endosome protein Rab17 regulates melanocytic filopodia formation and melanosome trafficking
Journal name Traffic   Check publisher's open access policy
ISSN 1398-9219
1600-0854
Publication date 2011-05
Sub-type Article (original research)
DOI 10.1111/j.1600-0854.2011.01172.x
Volume 12
Issue 5
Start page 627
End page 643
Total pages 17
Place of publication Malden, MA, United States
Publisher Wiley-Blackwell Publishing
Collection year 2012
Language eng
Formatted abstract
Background: C-reactive protein (CRP) is a predictor of cardiovascular risk. It circulates as a pentameric protein in plasma. Recently, a potential dissociation mechanism from the disc-shaped pentameric CRP (pCRP) into single monomers (monomeric or mCRP) has been described. It has been shown that mCRP has strong pro-inflammatory
effects on monocytes. To further define the role of mCRP in determining monocyte phenotype, the effects of CRP isoforms on THP-1 protein expression profiles were determined. The hypothesis to be tested was that mCRP induces specific changes in the protein expression profile of THP-1 cells that differ from that of pCRP.
Methods: Protein cell lysates from control and mCRP, pCRP or LPS-treated THP-1 cells were displayed using 2-dimensional SDS PAGE and compared. Differentially expressed proteins were identified by MALDI-TOF MS and confirmed by Western blotting.
Results: mCRP significantly up-regulates ubiquitin-activating enzyme E1, a member of the ubiquitin-proteasome system in THP-1 monocytes. Furthermore, HSP 70, alpha-actinin-4 (ACTN4) and alpha-enolase/enolase 1 were upregulated. The proteomic profile of LPS and pCRP treated monocytes differ significantly from that of mCRP.
Conclusion: The data obtained in this study support the hypothesis that isoform-specific effects of CRP may differentially regulate the phenotype of monocytes.
Keyword Filopodia
Melanin
Melanocyte
Melanosome
MITF
Rab11
Rab17
Recycling Endosome
Cultured Human Melanocytes
Myosin-Va
Griscelli-Syndrome
Epithelial-Cells
Membrane Trafficking
Cytokine Secretion
Melanin Transfer
Gene-Product
Cyclic-Amp
Rab27A
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Institute for Molecular Bioscience - Publications
 
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