Isolation and initial characterization of constitutive nitrate reductase-deficient mutants Nr328 and Nr345 of soybean (glycine-max)

Carroll, Bernard J. and Gresshoff, Peter M. (1986) Isolation and initial characterization of constitutive nitrate reductase-deficient mutants Nr328 and Nr345 of soybean (glycine-max). Plant Physiology, 81 2: 572-576. doi:10.1104/pp.81.2.572


Author Carroll, Bernard J.
Gresshoff, Peter M.
Title Isolation and initial characterization of constitutive nitrate reductase-deficient mutants Nr328 and Nr345 of soybean (glycine-max)
Journal name Plant Physiology   Check publisher's open access policy
ISSN 0032-0889
1532-2548
Publication date 1986-06
Sub-type Article (original research)
DOI 10.1104/pp.81.2.572
Volume 81
Issue 2
Start page 572
End page 576
Total pages 5
Place of publication Rockville, MD, United States
Publisher American Society of Plant Biologists
Language eng
Formatted abstract
Two nitrate reductase deficient mutants of soybean (Glycine max [L.] Merr. cv Bragg) were isolated from approximately 10,000 M2 seedlings, using a direct enzymic assay in microtiter plates. Stable inheritance of NR345 and NR328 phenotypes has been demonstrated through to the M5 generation. Both mutants were affected in constitutive nitrate reductase activity. Assayable activities of cNR in nitrate-free grown seedlings was about 3 to 4% of the control for NR345 and 14 to 16% of the control for NR328. Both mutants expressed inducible NR during early plant development and were sensitive to nitrate and urea inhibition of nodulation. These new mutants will allow an extension of the characterization of nitrate reductases and their function in soybean. Preliminary evidence indicates that NR345 is similar to the previously isolated mutant nr1, while NR328 is different.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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